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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

COL3A1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
COL3A1 N/A
Sequence Length (AA) Molecular Weight (Da)
1466 138564
Protein Name
Collagen alpha-1(III) chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMSFVQKGSW LLLALLHPTI ILAQQEAVEG GCSHLGQSYA DRDVWKPEPC
60 70 80 90 100
QICVCDSGSV LCDDIICDDQ ELDCPNPEIP FGECCAVCPQ PPTAPTRPPN
110 120 130 140 150
GQGPQGPKGD PGPPGIPGRN GDPGIPGQPG SPGSPGPPGI CESCPTGPQN
160 170 180 190 200
YSPQYDSYDV KSGVAVGGLA GYPGPAGPPG PPGPPGTSGH PGSPGSPGYQ
210 220 230 240 250
GPPGEPGQAG PSGPPGPPGA IGPSGPAGKD GESGRPGRPG ERGLPGPPGI
260 270 280 290 300
KGPAGIPGFP GMKGHRGFDG RNGEKGETGA PGLKGENGLP GENGAPGPMG
310 320 330 340 350
PRGAPGERGR PGLPGAAGAR GNDGARGSDG QPGPPGPPGT AGFPGSPGAK
360 370 380 390 400
GEVGPAGSPG SNGAPGQRGE PGPQGHAGAQ GPPGPPGING SPGGKGEMGP
410 420 430 440 450
AGIPGAPGLM GARGPPGPAG ANGAPGLRGG AGEPGKNGAK GEPGPRGERG
460 470 480 490 500
EAGIPGVPGA KGEDGKDGSP GEPGANGLPG AAGERGAPGF RGPAGPNGIP
510 520 530 540 550
GEKGPAGERG APGPAGPRGA AGEPGRDGVP GGPGMRGMPG SPGGPGSDGK
560 570 580 590 600
PGPPGSQGES GRPGPPGPSG PRGQPGVMGF PGPKGNDGAP GKNGERGGPG
610 620 630 640 650
GPGPQGPPGK NGETGPQGPP GPTGPGGDKG DTGPPGPQGL QGLPGTGGPP
660 670 680 690 700
GENGKPGEPG PKGDAGAPGA PGGKGDAGAP GERGPPGLAG APGLRGGAGP
710 720 730 740 750
PGPEGGKGAA GPPGPPGAAG TPGLQGMPGE RGGLGSPGPK GDKGEPGGPG
760 770 780 790 800
ADGVPGKDGP RGPTGPIGPP GPAGQPGDKG EGGAPGLPGI AGPRGSPGER
810 820 830 840 850
GETGPPGPAG FPGAPGQNGE PGGKGERGAP GEKGEGGPPG VAGPPGGSGP
860 870 880 890 900
AGPPGPQGVK GERGSPGGPG AAGFPGARGL PGPPGSNGNP GPPGPSGSPG
910 920 930 940 950
KDGPPGPAGN TGAPGSPGVS GPKGDAGQPG EKGSPGAQGP PGAPGPLGIA
960 970 980 990 1000
GITGARGLAG PPGMPGPRGS PGPQGVKGES GKPGANGLSG ERGPPGPQGL
1010 1020 1030 1040 1050
PGLAGTAGEP GRDGNPGSDG LPGRDGSPGG KGDRGENGSP GAPGAPGHPG
1060 1070 1080 1090 1100
PPGPVGPAGK SGDRGESGPA GPAGAPGPAG SRGAPGPQGP RGDKGETGER
1110 1120 1130 1140 1150
GAAGIKGHRG FPGNPGAPGS PGPAGQQGAI GSPGPAGPRG PVGPSGPPGK
1160 1170 1180 1190 1200
DGTSGHPGPI GPPGPRGNRG ERGSEGSPGH PGQPGPPGPP GAPGPCCGGV
1210 1220 1230 1240 1250
GAAAIAGIGG EKAGGFAPYY GDEPMDFKIN TDEIMTSLKS VNGQIESLIS
1260 1270 1280 1290 1300
PDGSRKNPAR NCRDLKFCHP ELKSGEYWVD PNQGCKLDAI KVFCNMETGE
1310 1320 1330 1340 1350
TCISANPLNV PRKHWWTDSS AEKKHVWFGE SMDGGFQFSY GNPELPEDVL
1360 1370 1380 1390 1400
DVHLAFLRLL SSRASQNITY HCKNSIAYMD QASGNVKKAL KLMGSNEGEF
1410 1420 1430 1440 1450
KAEGNSKFTY TVLEDGCTKH TGEWSKTVFE YRTRKAVRLP IVDIAPYDIG
1460 1466
GPDQEFGVDV GPVCFL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1180 Collapse assay details

Data source: Panorama

Official Gene Symbol
COL3A1
Peptide Sequence
GPVGPSGPPGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1140
Peptide End
1150
CPTAC ID
CPTAC-1180
Peptide Molecular Mass
948.5029
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM

CPTAC-1168:
FCER1G.
AAITSYEK 		CPTAC-1169:
MELK.
DLKPENLLFDEYHK 		CPTAC-1170:
PEPD.
VPLALFALNR
CPTAC-1171:
PES1.
LAALSASLAR 		CPTAC-1172:
PTPRC.
LFLAEFQSIPR 		CPTAC-1173:
SYK.
NYLGGFALSVAHGR
CPTAC-1174:
SYK.
LIATTAHEK 		CPTAC-1175:
ANKRD27.
LYDLPDEPFTR 		CPTAC-1176:
ASGR2.
ADHDALLFHLK
CPTAC-1177:
C3AR1.
STHC[+57.0]PSNNVISER 		CPTAC-1178:
CD79A.
SHGGIYVC[+57.0]R 		CPTAC-1179:
CEACAM1.
TIIVTELSPVVAKPQIK
CPTAC-1180:
COL3A1.
GPVGPSGPPGK 		CPTAC-1181:
CYBB.
LLGSALALAR 		CPTAC-1182:
EVI2B.
STLLADDLEIK
CPTAC-1183:
FCGR2B.
VTFFQNGK 		CPTAC-1184:
GPR20.
YLAIVRPEGSR 		CPTAC-1185:
SIX6.
AIVAFHGGNYR
CPTAC-1186:
THBS2.
GTLLALEGPGLSQR 		CPTAC-1187:
TOP2A.
TLAVSGLGVVGR 		CPTAC-1188:
TSPAN14.
TLISDIEAVK
CPTAC-1189:
TLE1.
FTIPESLDR 		CPTAC-1190:
SLC7A9.
LGSYVQNIFTAAK 		CPTAC-1191:
SLC7A9.
VLSYISVR
CPTAC-1192:
PEPD.
FEVNNTILHPEIVEC[+57.0]R 		CPTAC-1193:
PES1.
FLLHEPIVNK 		CPTAC-1194:
TARS.
AELNPWPEYIYTR
CPTAC-1195:
TARS.
GFQEVVTPNIFNSR 		CPTAC-1196:
STARD3NL.
VLILAYAVC[+57.0]R 		CPTAC-1197:
STARD3NL.
LLIVQDASER
CPTAC-1198:
ANKRD27.
LNDPSVVTPFSR 		CPTAC-1199:
CD48.
LQVLDPVPKPVIK 		CPTAC-1200:
CDK1.
LESEEEGVPSTAIR
CPTAC-1201:
COL11A1.
GFDGLPGLPGDK 		CPTAC-1202:
COL11A1.
GEVGQIGPR 		CPTAC-1203:
CYBB.
IGVFLC[+57.0]GPEALAETLSK
CPTAC-1204:
DLGAP5.
DVNIPTLEGR 		CPTAC-1205:
DLGAP5.
IAQPHHGVPYFR 		CPTAC-1206:
ETS1.
YENDYPSVILR
CPTAC-1207:
EVI2B.
STPGFILDTTSNK 		CPTAC-1208:
LYL1.
DQAAALAAGPTPPGPR 		CPTAC-1209:
LYL1.
SQPAPPADPDGSPGGAARPIK
CPTAC-1210:
MCM10.
GTNLIIQETR 		CPTAC-1211:
MCM10.
IGGETLLPR 		CPTAC-1212:
OSGIN1.
AVDDPGLVFNQLPK
CPTAC-1213:
PIK3CD.
GELLNPTGTVR 		CPTAC-1214:
SFTPA1.
NPEENEAIASFVK 		CPTAC-1215:
ZNF507.
TKPDPLIHVIQK
CPTAC-1216:
ZNF592.
AAPLIVEVFNK 		CPTAC-1217:
ANXA11.
TPVLFDIYEIK 		CPTAC-1218:
ANXA11.
DAQELYAAGENR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 29 13 14.2 27.5 15.8 14.8 40 20.5 20.5 15 15 15
y7 (1+) 22.9 7.4 9.6 22 15.1 11.7 31.8 16.8 15.1 15 15 15
y8 (1+) 13.1 4.9 8.9 16.2 8.5 8.9 20.8 9.8 12.6 15 15 15
sum 8.9 5.1 6.4 9.2 9.3 7.5 12.8 10.6 9.9 15 15 15

Selectivity

Data source: Panorama

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Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y8 (1+) 5.93 2.46 8.64 5.51 1.99 6.82
y7 (1+) 5.82 2.51 8.07 6.03 2.1 5.34
y4 (1+) 6.01 2.52 8.89 6.88 2.26 7.48
sum 5.86 2.48 8.5 5.82 2.06 6.31

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y7 (1+) 6.8 9.6 6 13.7 7
y4 (1+) 41.3 24.2 6.2 16.7 13.8
y8 (1+) 13.2 7.9 2.8 4.8 10.5
sum 5.1 3.2 2.8 4.2 4.3
Fragment ion / Transition all_intra_CV all_inter_CV
y7 (1+) 8.8 9.4
y4 (1+) 21.1 19.5
y8 (1+) 7.9 8.4
sum 3.8 5.7

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
y7 (1+) 46.3 62.6 77.9
y4 (1+) 59.8 73.1 94.4
y8 (1+) 54.7 47.8 72.6
sum 46.8 48.2 67.2

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3364 Collapse assay details

Data source: Panorama

Official Gene Symbol
Col3a1
Peptide Sequence
GGAGPPGPEGGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
695
Peptide End
706
CPTAC ID
non-CPTAC-3364
Peptide Molecular Mass
979.4723
Species
Mus musculus (Mouse)
Assay Type
Direct MRM or SRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM

CPTAC-1168:
FCER1G.
AAITSYEK 		CPTAC-1169:
MELK.
DLKPENLLFDEYHK 		CPTAC-1170:
PEPD.
VPLALFALNR
CPTAC-1171:
PES1.
LAALSASLAR 		CPTAC-1172:
PTPRC.
LFLAEFQSIPR 		CPTAC-1173:
SYK.
NYLGGFALSVAHGR
CPTAC-1174:
SYK.
LIATTAHEK 		CPTAC-1175:
ANKRD27.
LYDLPDEPFTR 		CPTAC-1176:
ASGR2.
ADHDALLFHLK
CPTAC-1177:
C3AR1.
STHC[+57.0]PSNNVISER 		CPTAC-1178:
CD79A.
SHGGIYVC[+57.0]R 		CPTAC-1179:
CEACAM1.
TIIVTELSPVVAKPQIK
CPTAC-1180:
COL3A1.
GPVGPSGPPGK 		CPTAC-1181:
CYBB.
LLGSALALAR 		CPTAC-1182:
EVI2B.
STLLADDLEIK
CPTAC-1183:
FCGR2B.
VTFFQNGK 		CPTAC-1184:
GPR20.
YLAIVRPEGSR 		CPTAC-1185:
SIX6.
AIVAFHGGNYR
CPTAC-1186:
THBS2.
GTLLALEGPGLSQR 		CPTAC-1187:
TOP2A.
TLAVSGLGVVGR 		CPTAC-1188:
TSPAN14.
TLISDIEAVK
CPTAC-1189:
TLE1.
FTIPESLDR 		CPTAC-1190:
SLC7A9.
LGSYVQNIFTAAK 		CPTAC-1191:
SLC7A9.
VLSYISVR
CPTAC-1192:
PEPD.
FEVNNTILHPEIVEC[+57.0]R 		CPTAC-1193:
PES1.
FLLHEPIVNK 		CPTAC-1194:
TARS.
AELNPWPEYIYTR
CPTAC-1195:
TARS.
GFQEVVTPNIFNSR 		CPTAC-1196:
STARD3NL.
VLILAYAVC[+57.0]R 		CPTAC-1197:
STARD3NL.
LLIVQDASER
CPTAC-1198:
ANKRD27.
LNDPSVVTPFSR 		CPTAC-1199:
CD48.
LQVLDPVPKPVIK 		CPTAC-1200:
CDK1.
LESEEEGVPSTAIR
CPTAC-1201:
COL11A1.
GFDGLPGLPGDK 		CPTAC-1202:
COL11A1.
GEVGQIGPR 		CPTAC-1203:
CYBB.
IGVFLC[+57.0]GPEALAETLSK
CPTAC-1204:
DLGAP5.
DVNIPTLEGR 		CPTAC-1205:
DLGAP5.
IAQPHHGVPYFR 		CPTAC-1206:
ETS1.
YENDYPSVILR
CPTAC-1207:
EVI2B.
STPGFILDTTSNK 		CPTAC-1208:
LYL1.
DQAAALAAGPTPPGPR 		CPTAC-1209:
LYL1.
SQPAPPADPDGSPGGAARPIK
CPTAC-1210:
MCM10.
GTNLIIQETR 		CPTAC-1211:
MCM10.
IGGETLLPR 		CPTAC-1212:
OSGIN1.
AVDDPGLVFNQLPK
CPTAC-1213:
PIK3CD.
GELLNPTGTVR 		CPTAC-1214:
SFTPA1.
NPEENEAIASFVK 		CPTAC-1215:
ZNF507.
TKPDPLIHVIQK
CPTAC-1216:
ZNF592.
AAPLIVEVFNK 		CPTAC-1217:
ANXA11.
TPVLFDIYEIK 		CPTAC-1218:
ANXA11.
DAQELYAAGENR

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 10.6 12.3 11.6 16.4 14.7 13.6 19.5 19.2 17.9 15 15 15
y8 (2+) 7.3 4.9 6.4 13.5 10.1 10.1 15.3 11.2 12 15 15 15
y9 (2+) 15.6 10.6 6.3 21.5 14.3 10 26.6 17.8 11.8 15 15 15
y7 (1+) 9.6 8.4 8.6 20.1 13.1 9.4 22.3 15.6 12.7 15 15 15
y8 (1+) 9.7 8.5 7.1 16.2 15.6 9.9 18.9 17.8 12.2 15 15 15
sum 4.4 4.2 4.5 14.9 10.5 7.8 15.5 11.3 9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments