What is “CPTAC Characterized?”

CPTAC characterization is a set of experiments designed to help users evaluate assay performance before investing time, money, and energy into adopting and deploying the assays in their own laboratories. Assays in the portal are generally comprised of “Tier 2” assays as defined by recommendations for “fit-for-purpose” validation of targeted proteomics assays.* There are five recommended experiments (shown below). Each experiment is described in detail in the Assay Characterization Guidance Document (available for download here). Experiments 1 and 2 are required for upload of assays into the portal. Other experiments are optional but encouraged.

* Targeted peptide measurements in biology and medicine: best practices for mass spectrometry-based assay development using a fit-for-purpose approach. Carr SA, Abbatiello SE, Ackermann BL, Borchers C, Domon B, Deutsch EW, Grant RP, Hoofnagle AN, Hüttenhain R, Koomen JM, Liebler DC, Liu T, MacLean B, Mani DR, Mansfield E, Neubert H, Paulovich AG, Reiter L, Vitek O, Aebersold R, Anderson L, Bethem R, Blonder J, Boja E, Botelho J, Boyne M, Bradshaw RA, Burlingame AL, Chan D, Keshishian H, Kuhn E, Kinsinger C, Lee JS, Lee SW, Moritz R, Oses-Prieto J, Rifai N, Ritchie J, Rodriguez H, Srinivas PR, Townsend RR, Van Eyk J, Whiteley G, Wiita A, Weintraub S. Mol Cell Proteomics. 2014 Mar;13(3):907-17. doi: 10.1074/mcp.M113.036095. PMID: 24443746

CPTAC Characterization: 5 Recommended Experiments

How to Cite the CPTAC Assay Portal in Your Publications

When referring to the website, please cite:
CPTAC Assay Portal: a repository of targeted proteomic assays. Whiteaker JR, Halusa GN, Hoofnagle AN, Sharma V, MacLean B, Yan P, Wrobel JA, Kennedy J, Mani DR, Zimmerman LJ, Meyer MR, Mesri M, Rodriguez H; Clinical Proteomic Tumor Analysis Consortium (CPTAC), Paulovich AG. Nat Methods. 2014 Jul;11(7):703-4. doi: 10.1038/nmeth.3002. PMID: 24972168
When using or referencing the CPTAC Assay Portal in general, please cite the main page:
When using, reporting, referencing, or redistributing individual assays, please cite the specific assay by ID number. For example:
Submitting References
To submit references using the assays found on the portal, visit the “Contact Us” page.
The following is a list of highlighted publications using the CPTAC Assay Portal:
Coming soon

How to Provide Feedback on Individual Assays

The CPTAC Assay Portal enables users to provide comments on individual assays in order to help the broad community of researchers realize success. What was your experience with a given assay? Does it perform in a similar manner under different conditions? Did you alter the method to improve performance? You can provide feedback and read others’ comments below the assay details. To find comments, from the “Gene / Protein” page, click on an assay of interest and scroll to the bottom of the page. The Assay Portal uses Disqus to provide the framework for commenting. Create an account with Disqus, or login using Facebook, Twitter, or Google account to get started.

How to Contribute Assays to the Portal

Coming soon…

How to Provide Feedback on the Assay Portal

Visit “our “Contact Us”” page located under ““Stay Connected”” on the right panel of the Assay Portal website pages.

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