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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PIGR

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PIGR N/A
Sequence Length (AA) Molecular Weight (Da)
764 83284
Protein Name
Polymeric immunoglobulin receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLLFVLTCLL AVFPAISTKS PIFGPEEVNS VEGNSVSITC YYPPTSVNRH
60 70 80 90 100
TRKYWCRQGA RGGCITLISS EGYVSSKYAG RANLTNFPEN GTFVVNIAQL
110 120 130 140 150
SQDDSGRYKC GLGINSRGLS FDVSLEVSQG PGLLNDTKVY TVDLGRTVTI
160 170 180 190 200
NCPFKTENAQ KRKSLYKQIG LYPVLVIDSS GYVNPNYTGR IRLDIQGTGQ
210 220 230 240 250
LLFSVVINQL RLSDAGQYLC QAGDDSNSNK KNADLQVLKP EPELVYEDLR
260 270 280 290 300
GSVTFHCALG PEVANVAKFL CRQSSGENCD VVVNTLGKRA PAFEGRILLN
310 320 330 340 350
PQDKDGSFSV VITGLRKEDA GRYLCGAHSD GQLQEGSPIQ AWQLFVNEES
360 370 380 390 400
TIPRSPTVVK GVAGGSVAVL CPYNRKESKS IKYWCLWEGA QNGRCPLLVD
410 420 430 440 450
SEGWVKAQYE GRLSLLEEPG NGTFTVILNQ LTSRDAGFYW CLTNGDTLWR
460 470 480 490 500
TTVEIKIIEG EPNLKVPGNV TAVLGETLKV PCHFPCKFSS YEKYWCKWNN
510 520 530 540 550
TGCQALPSQD EGPSKAFVNC DENSRLVSLT LNLVTRADEG WYWCGVKQGH
560 570 580 590 600
FYGETAAVYV AVEERKAAGS RDVSLAKADA APDEKVLDSG FREIENKAIQ
610 620 630 640 650
DPRLFAEEKA VADTRDQADG SRASVDSGSS EEQGGSSRAL VSTLVPLGLV
660 670 680 690 700
LAVGAVAVGV ARARHRKNVD RVSIRSYRTD ISMSDFENSR EFGANDNMGA
710 720 730 740 750
SSITQETSLG GKEEFVATTE STTETKEPKK AKRSSKEEAE MAYKDFLLQS
760 764
STVAAEAQDG PQEA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-685 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIGR
Peptide Modified Sequence
VPGN[+1.0]VTAVLGETLK
Modification Type
Deamidated (NQ)
Protein - Site of Modification
469
Peptide - Site of Modification
4
Peptide Start
466
Peptide End
479
CPTAC ID
CPTAC-685
Peptide Molecular Mass
1,397.7766
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM-MS
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 7.8 5.1 4.9 7.7 5.8 5.4 11 7.7 7.3 15 15 15
y9 (1+) 3.4 4.9 3.4 3.9 4.8 4.9 5.2 6.9 6 15 15 15
y8 (1+) 4.6 4.7 3 6.6 4.8 4.8 8 6.7 5.7 15 15 15
sum 4.1 4.6 2.9 4.5 4.4 4.3 6.1 6.4 5.2 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-6117 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIGR
Peptide Sequence
VYTVDLGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
139
Peptide End
146
CPTAC ID
non-CPTAC-6117
Peptide Molecular Mass
921.4920
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6.2 6.5 7.7 6.5 6.9 5.8 9 9.5 9.6 16 16 17
y4 (1+) 8.6 4.6 7.1 8.7 5.4 6.4 12.2 7.1 9.6 16 16 17
y5 (1+) 13.1 9.7 6.3 12.2 11 7.1 17.9 14.7 9.5 16 16 17
y6 (1+) 6.1 9.3 4.6 7.2 10.3 5.5 9.4 13.9 7.2 16 16 17
y7 (1+) 15.8 5.6 9.9 14.1 5.1 13 21.2 7.6 16.3 16 16 17
sum 4.9 4.9 3.2 4.9 5.3 3 6.9 7.2 4.4 16 16 17

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
b2 (1+) 12.1 6 18.1 4.9 8.4
y4 (1+) 9.3 9 9.8 12.8 3.8
y5 (1+) 8.2 6.8 19.6 10.1 13.5
y6 (1+) 7.3 5.5 15.4 6.5 13.9
y7 (1+) 7.4 10.6 13.2 5 4.8
sum 4.5 2.3 5.8 2.5 9.2
Fragment ion / Transition all_intra_CV all_inter_CV
b2 (1+) 9.2 10.3
y4 (1+) 8.9 7.8
y5 (1+) 10.8 11.7
y6 (1+) 9 9
y7 (1+) 8.6 8.4
sum 4.4 3.4

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
b2 (1+) 74.5 52.5 91.1
y4 (1+) 44.2 73.4 85.7
y5 (1+) 43.4 87.3 97.5
y6 (1+) 14.5 24.4 28.4
y7 (1+) 22 117.7 119.7
sum 1.5 33.4 33.4

Additional Resources and Comments

Resources
Download SOPs
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Comments

Assay Details for non-CPTAC-3738 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pigr
Peptide Sequence
VALEEEVVDSSISEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
585
Peptide End
599
CPTAC ID
non-CPTAC-3738
Peptide Molecular Mass
1,632.8094
Species
Mus musculus (Mouse)
Assay Type
Direct MRM-MS
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 17 11.4 6.9 16 12.6 8.4 23.3 17 10.9 15 15 15
y3 (1+) 12.7 6.7 4.3 13.5 7.8 5 18.5 10.3 6.6 15 15 15
y7 (1+) 17.5 6.5 4 15.9 8.1 8.8 23.6 10.4 9.7 15 15 15
y8 (1+) 10.4 5.2 6.5 11.3 6.3 5.7 15.4 8.2 8.6 15 15 15
y9 (1+) 13.6 8.1 8.5 12.1 10.3 10 18.2 13.1 13.1 15 15 15
sum 9.1 2.3 1.8 7.2 4.4 4.1 11.6 5 4.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments