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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

PIK3CB

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PIK3CB PIK3C1
Sequence Length (AA) Molecular Weight (Da)
1070 122762
Protein Name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MCFSFIMPPA MADILDIWAV DSQIASDGSI PVDFLLPTGI YIQLEVPREA
60 70 80 90 100
TISYIKQMLW KQVHNYPMFN LLMDIDSYMF ACVNQTAVYE ELEDETRRLC
110 120 130 140 150
DVRPFLPVLK LVTRSCDPGE KLDSKIGVLI GKGLHEFDSL KDPEVNEFRR
160 170 180 190 200
KMRKFSEEKI LSLVGLSWMD WLKQTYPPEH EPSIPENLED KLYGGKLIVA
210 220 230 240 250
VHFENCQDVF SFQVSPNMNP IKVNELAIQK RLTIHGKEDE VSPYDYVLQV
260 270 280 290 300
SGRVEYVFGD HPLIQFQYIR NCVMNRALPH FILVECCKIK KMYEQEMIAI
310 320 330 340 350
EAAINRNSSN LPLPLPPKKT RIISHVWENN NPFQIVLVKG NKLNTEETVK
360 370 380 390 400
VHVRAGLFHG TELLCKTIVS SEVSGKNDHI WNEPLEFDIN ICDLPRMARL
410 420 430 440 450
CFAVYAVLDK VKTKKSTKTI NPSKYQTIRK AGKVHYPVAW VNTMVFDFKG
460 470 480 490 500
QLRTGDIILH SWSSFPDELE EMLNPMGTVQ TNPYTENATA LHVKFPENKK
510 520 530 540 550
QPYYYPPFDK IIEKAAEIAS SDSANVSSRG GKKFLPVLKE ILDRDPLSQL
560 570 580 590 600
CENEMDLIWT LRQDCREIFP QSLPKLLLSI KWNKLEDVAQ LQALLQIWPK
610 620 630 640 650
LPPREALELL DFNYPDQYVR EYAVGCLRQM SDEELSQYLL QLVQVLKYEP
660 670 680 690 700
FLDCALSRFL LERALGNRRI GQFLFWHLRS EVHIPAVSVQ FGVILEAYCR
710 720 730 740 750
GSVGHMKVLS KQVEALNKLK TLNSLIKLNA VKLNRAKGKE AMHTCLKQSA
760 770 780 790 800
YREALSDLQS PLNPCVILSE LYVEKCKYMD SKMKPLWLVY NNKVFGEDSV
810 820 830 840 850
GVIFKNGDDL RQDMLTLQML RLMDLLWKEA GLDLRMLPYG CLATGDRSGL
860 870 880 890 900
IEVVSTSETI ADIQLNSSNV AAAAAFNKDA LLNWLKEYNS GDDLDRAIEE
910 920 930 940 950
FTLSCAGYCV ASYVLGIGDR HSDNIMVKKT GQLFHIDFGH ILGNFKSKFG
960 970 980 990 1000
IKRERVPFIL TYDFIHVIQQ GKTGNTEKFG RFRQCCEDAY LILRRHGNLF
1010 1020 1030 1040 1050
ITLFALMLTA GLPELTSVKD IQYLKDSLAL GKSEEEALKQ FKQKFDEALR
1060 1070
ESWTTKVNWM AHTVRKDYRS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6118 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3CB
Peptide Sequence
AAEIASSDSANVSSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
515
Peptide End
529
CPTAC ID
non-CPTAC-6118
Peptide Molecular Mass
1,463.6852
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 17.7 4.2 19.9 22.1 3.9 22.8 28.3 5.7 30.3 15 16 17
y10 (1+) 16.4 7.5 6.9 20.2 6.6 5.6 26 10 8.9 15 16 17
y11 (1+) 14.8 4.1 7.1 16.7 5.2 6.1 22.3 6.6 9.4 15 16 17
y3 (1+) 13.6 7 6.6 18.8 8.3 6.5 23.2 10.9 9.3 15 16 17
y9 (1+) 14.2 5.1 5.5 16.4 8.4 5 21.7 9.8 7.4 15 16 17
sum 13 3 5.2 16.1 2.1 4.1 20.7 3.7 6.6 15 16 17

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
b3 (1+) 4.7 5.4 3.5 3.1 8.9
y10 (1+) 4.6 2.3 5.3 4.6 2.1
y11 (1+) 7.3 8 6.9 8.1 17.6
y3 (1+) 10.6 7.4 5.9 13.3 7
y9 (1+) 5.5 9.2 6 9.2 12.9
sum 2 1.4 2.3 3.9 5.2
Fragment ion / Transition all_intra_CV all_inter_CV
b3 (1+) 5.2 5
y10 (1+) 3.5 4.1
y11 (1+) 9.3 8.8
y3 (1+) 8.6 9.4
y9 (1+) 8.7 7.3
sum 2.7 3

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
b3 (1+) 7.8 10.3 12.9
y10 (1+) 9.8 8.8 13.2
y11 (1+) 7.7 7.9 11
y3 (1+) 5.4 19.6 20.3
y9 (1+) 4.4 20.9 21.4
sum 0.3 5.3 5.3

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-6119 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3CB
Peptide Sequence
EAGLDLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
829
Peptide End
835
CPTAC ID
non-CPTAC-6119
Peptide Molecular Mass
772.4079
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama

view larger image
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view larger image

Response Curves

Data source: Panorama

view larger image
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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 8.6 4.4 6.1 10.7 3.5 5 13.7 5.6 7.9 16 16 17
y3 (1+) 8.8 3.3 5.8 10 3.5 5.5 13.3 4.8 8 16 16 17
y5 (1+) 10 5.5 1.8 10.2 7.1 3.7 14.3 9 4.1 16 16 17
y4 (2+) 52.9 11.2 4 51.9 12.2 4 74.1 16.6 5.7 16 16 17
y5 (2+) 21 4.8 4.8 20.3 4.7 4 29.2 6.7 6.2 16 16 17
sum 5.5 2.1 2.8 6.2 1.9 3.2 8.3 2.8 4.3 16 16 17

Stability

Data source: Panorama

view larger image
Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
Fragment ion / Transition all_intra_CV all_inter_CV

Endogenous

Data source: Panorama

view larger image
Fragment ion / Transition intra_CV inter_CV total_CV
y2 (1+) 2.7 46.5 46.6
y3 (1+) 20.1 34.9 40.3
y5 (1+) 6.6 18.9 20
y4 (2+) 15.5 49.8 52.2
y5 (2+) 44.9 67.2 80.8
sum 9.9 18.6 21.1

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3132 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3CB
Peptide Sequence
IGQFLFWHLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
670
Peptide End
679
CPTAC ID
CPTAC-3132
Peptide Molecular Mass
1,315.7190
Species
Homo Sapiens
Assay Type
Direct MRM-MS
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 5.3 3.4 4.1 5.4 7.4 7 7.6 8.1 8.1 15 15 15
y5 (1+) 5.5 3.5 4.9 5 7.7 6.9 7.4 8.5 8.5 15 15 15
y3 (1+) 4.4 2.9 4.4 4.8 6.4 6.3 6.5 7 7.7 15 15 15
sum 4.4 3.2 4.5 4.5 7.2 6.7 6.3 7.9 8.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3131 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3CB
Peptide Modified Sequence
LC[+57.0]FAVYAVLDK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
402
Peptide - Site of Modification
2
Peptide Start
400
Peptide End
410
CPTAC ID
CPTAC-3131
Peptide Molecular Mass
1,297.6740
Species
Homo Sapiens
Assay Type
Direct MRM-MS
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 20.6 6.1 9.7 32 9.2 21.6 38.1 11 23.7 15 15 15
y6 (1+) 15.4 4.7 9 25.5 10.8 18.4 29.8 11.8 20.5 15 15 15
y9 (1+) 13.3 3.9 4.9 20.1 7.5 17.3 24.1 8.5 18 15 15 15
sum 13.3 3 6.9 22.8 7.4 18.2 26.4 8 19.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3130 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3CB
Peptide Sequence
VFGEDSVGVIFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
794
Peptide End
805
CPTAC ID
CPTAC-3130
Peptide Molecular Mass
1,295.6762
Species
Homo Sapiens
Assay Type
Direct MRM-MS
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 14.4 5 6.5 16.8 8.7 7.6 22.1 10 10 15 15 15
y3 (1+) 10.4 4.5 5.4 12.7 9 8.2 16.4 10.1 9.8 15 15 15
y10 (1+) 13 5.8 6.8 16 11 8.4 20.6 12.4 10.8 15 15 15
sum 11.9 5.3 6.2 14.5 10 8 18.8 11.3 10.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments