Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

RFTN1

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
RFTN1 KIAA0084
Sequence Length (AA) Molecular Weight (Da)
578 63146
Protein Name
Raftlin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGCGLNKLEK RDEKRPGNIY STLKRPQVET KIDVSYEYRF LEFTTLSAAE
60 70 80 90 100
LPGSSAVRLA SLRDLPAQLL ELYQQGFSLA ALHPFVQPTH EREKTPLEHI
110 120 130 140 150
FRAILIKKTD RSQKTDLHNE GYILELDCCS SLDHPTDQKL IPEFIKKIQE
160 170 180 190 200
AASQGLKFVG VIPQYHSSVN SAGSSAPVST ANSTEDARDA KNARGDHASL
210 220 230 240 250
ENEKPGTGDV CSAPAGRNQS PEPSSGPRGE VPLAKQPSSP SGEGDGGELS
260 270 280 290 300
PQGVSKTLDG PESNPLEVHE EPLSGKMEIF TLFNKPKSHQ KCRQYYPVTI
310 320 330 340 350
PLHVSKNGQT VSGLDANWLE HMSDHFRKGG MLVNAVFYLG IVNDSLHGLT
360 370 380 390 400
DGVFIFEAVS TEDSKTIQGY DAIVVEQWTV LEGVEVQTDY VPLLNSLAAY
410 420 430 440 450
GWQLTCVLPT PVVKTTSEGS VSTKQIVFLQ RPCLPQKIKK KESKFQWRFS
460 470 480 490 500
REEMHNRQMR KSKGKLSARD KQQAEENEKN LEDQSSKAGD MGNCVSGQQQ
510 520 530 540 550
EGGVSEEMKG PVQEDKGEQL SPGGLLCGVG VEGEAVQNGP ASHSRALVGI
560 570 578
CTGHSNPGED ARDGDAEEVR ELGTVEEN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1000 Collapse assay details

Data source: Panorama

Official Gene Symbol
RFTN1
Peptide Modified Sequence
GDHAS[+80.0]LENEKPGTGDVC[+57.0]SAPAGR
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
199, 211
Peptide - Site of Modification
5, 17
Peptide Start
195
Peptide End
217
CPTAC ID
CPTAC-1000
Peptide Molecular Mass
2,404.0115
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 27.5 15.8 36.2 30 21.4 45.4 40.7 26.6 58.1 15 24 15
y13 (2+) 32.4 10 9.4 20.1 10.3 9.9 38.1 14.4 13.7 15 24 15
y20-98 (2+) 28.9 14.8 18.2 17.4 17.1 20.3 33.7 22.6 27.3 15 24 15
sum 28.6 8.8 12.6 16.8 9.1 15.1 33.2 12.7 19.7 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments