SRRM2, CPTAC-1008 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SRRM2	KIAA0324, SRL300, SRM300

Sequence Length (AA)	Molecular Weight (Da)
2752	299615

Protein Name
Serine/arginine repetitive matrix protein 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MYNGIGLPTP	RGSGTNGYVQ	RNLSLVRGRR	GERPDYKGEE	ELRRLEAALV
60	70	80	90	100
KRPNPDILDH	ERKRRVELRC	LELEEMMEEQ	GYEEQQIQEK	VATFRLMLLE
110	120	130	140	150
KDVNPGGKEE	TPGQRPAVTE	THQLAELNEK	KNERLRAAFG	ISDSYVDGSS
160	170	180	190	200
FDPQRRAREA	KQPAPEPPKP	YSLVRESSSS	RSPTPKQKKK	KKKKDRGRRS
210	220	230	240	250
ESSSPRRERK	KSSKKKKHRS	ESESKKRKHR	SPTPKSKRKS	KDKKRKRSRS
260	270	280	290	300
TTPAPKSRRA	HRSTSADSAS	SSDTSRSRSR	SAAAKTHTTA	LAGRSPSPAS
310	320	330	340	350
GRRGEGDAPF	SEPGTTSTQR	PSSPETATKQ	PSSPYEDKDK	DKKEKSATRP
360	370	380	390	400
SPSPERSSTG	PEPPAPTPLL	AERHGGSPQP	LATTPLSQEP	VNPPSEASPT
410	420	430	440	450
RDRSPPKSPE	KLPQSSSSES	SPPSPQPTKV	SRHASSSPES	PKPAPAPGSH
460	470	480	490	500
REISSSPTSK	NRSHGRAKRD	KSHSHTPSRR	MGRSRSPATA	KRGRSRSRTP
510	520	530	540	550
TKRGHSRSRS	PQWRRSRSAQ	RWGRSRSPQR	RGRSRSPQRP	GWSRSRNTQR
560	570	580	590	600
RGRSRSARRG	RSHSRSPATR	GRSRSRTPAR	RGRSRSRTPA	RRRSRSRTPT
610	620	630	640	650
RRRSRSRTPA	RRGRSRSRTP	ARRRSRTRSP	VRRRSRSRSP	ARRSGRSRSR
660	670	680	690	700
TPARRGRSRS	RTPARRGRSR	SRTPARRSGR	SRSRTPARRG	RSRSRTPRRG
710	720	730	740	750
RSRSRSLVRR	GRSHSRTPQR	RGRSGSSSER	KNKSRTSQRR	SRSNSSPEMK
760	770	780	790	800
KSRISSRRSR	SLSSPRSKAK	SRLSLRRSLS	GSSPCPKQKS	QTPPRRSRSG
810	820	830	840	850
SSQPKAKSRT	PPRRSRSSSS	PPPKQKSKTP	SRQSHSSSSP	HPKVKSGTPP
860	870	880	890	900
RQGSITSPQA	NEQSVTPQRR	SCFESSPDPE	LKSRTPSRHS	CSGSSPPRVK
910	920	930	940	950
SSTPPRQSPS	RSSSPQPKVK	AIISPRQRSH	SGSSSPSPSR	VTSRTTPRRS
960	970	980	990	1000
RSVSPCSNVE	SRLLPRYSHS	GSSSPDTKVK	PETPPRQSHS	GSISPYPKVK
1010	1020	1030	1040	1050
AQTPPGPSLS	GSKSPCPQEK	SKDSLVQSCP	GSLSLCAGVK	SSTPPGESYF
1060	1070	1080	1090	1100
GVSSLQLKGQ	SQTSPDHRSD	TSSPEVRQSH	SESPSLQSKS	QTSPKGGRSR
1110	1120	1130	1140	1150
SSSPVTELAS	RSPIRQDRGE	FSASPMLKSG	MSPEQSRFQS	DSSSYPTVDS
1160	1170	1180	1190	1200
NSLLGQSRLE	TAESKEKMAL	PPQEDATASP	PRQKDKFSPF	PVQDRPESSL
1210	1220	1230	1240	1250
VFKDTLRTPP	RERSGAGSSP	ETKEQNSALP	TSSQDEELME	VVEKSEEPAG
1260	1270	1280	1290	1300
QILSHLSSEL	KEMSTSNFES	SPEVEERPAV	SLTLDQSQSQ	ASLEAVEVPS
1310	1320	1330	1340	1350
MASSWGGPHF	SPEHKELSNS	PLRENSFGSP	LEFRNSGPLG	TEMNTGFSSE
1360	1370	1380	1390	1400
VKEDLNGPFL	NQLETDPSLD	MKEQSTRSSG	HSSSELSPDA	VEKAGMSSNQ
1410	1420	1430	1440	1450
SISSPVLDAV	PRTPSRERSS	SASSPEMKDG	LPRTPSRRSR	SGSSPGLRDG
1460	1470	1480	1490	1500
SGTPSRHSLS	GSSPGMKDIP	RTPSRGRSEC	DSSPEPKALP	QTPRPRSRSP
1510	1520	1530	1540	1550
SSPELNNKCL	TPQRERSGSE	SSVDQKTVAR	TPLGQRSRSG	SSQELDVKPS
1560	1570	1580	1590	1600
ASPQERSESD	SSPDSKAKTR	TPLRQRSRSG	SSPEVDSKSR	LSPRRSRSGS
1610	1620	1630	1640	1650
SPEVKDKPRA	APRAQSGSDS	SPEPKAPAPR	ALPRRSRSGS	SSKGRGPSPE
1660	1670	1680	1690	1700
GSSSTESSPE	HPPKSRTARR	GSRSSPEPKT	KSRTPPRRRS	SRSSPELTRK
1710	1720	1730	1740	1750
ARLSRRSRSA	SSSPETRSRT	PPRHRRSPSV	SSPEPAEKSR	SSRRRRSASS
1760	1770	1780	1790	1800
PRTKTTSRRG	RSPSPKPRGL	QRSRSRSRRE	KTRTTRRRDR	SGSSQSTSRR
1810	1820	1830	1840	1850
RQRSRSRSRV	TRRRRGGSGY	HSRSPARQES	SRTSSRRRRG	RSRTPPTSRK
1860	1870	1880	1890	1900
RSRSRTSPAP	WKRSRSRASP	ATHRRSRSRT	PLISRRRSRS	RTSPVSRRRS
1910	1920	1930	1940	1950
RSRTSVTRRR	SRSRASPVSR	RRSRSRTPPV	TRRRSRSRTP	TTRRRSRSRT
1960	1970	1980	1990	2000
PPVTRRRSRS	RTPPVTRRRS	RSRTSPITRR	RSRSRTSPVT	RRRSRSRTSP
2010	2020	2030	2040	2050
VTRRRSRSRT	SPVTRRRSRS	RTPPAIRRRS	RSRTPLLPRK	RSRSRSPLAI
2060	2070	2080	2090	2100
RRRSRSRTPR	TARGKRSLTR	SPPAIRRRSA	SGSSSDRSRS	ATPPATRNHS
2110	2120	2130	2140	2150
GSRTPPVALN	SSRMSCFSRP	SMSPTPLDRC	RSPGMLEPLG	SSRTPMSVLQ
2160	2170	2180	2190	2200
QAGGSMMDGP	GPRIPDHQRT	SVPENHAQSR	IALALTAISL	GTARPPPSMS
2210	2220	2230	2240	2250
AAGLAARMSQ	VPAPVPLMSL	RTAPAANLAS	RIPAASAAAM	NLASARTPAI
2260	2270	2280	2290	2300
PTAVNLADSR	TPAAAAAMNL	ASPRTAVAPS	AVNLADPRTP	TAPAVNLAGA
2310	2320	2330	2340	2350
RTPAALAALS	LTGSGTPPTA	ANYPSSSRTP	QAPASANLVG	PRSAHATAPV
2360	2370	2380	2390	2400
NIAGSRTAAA	LAPASLTSAR	MAPALSGANL	TSPRVPLSAY	ERVSGRTSPP
2410	2420	2430	2440	2450
LLDRARSRTP	PSAPSQSRMT	SERAPSPSSR	MGQAPSQSLL	PPAQDQPRSP
2460	2470	2480	2490	2500
VPSAFSDQSR	CLIAQTTPVA	GSQSLSSGAV	ATTTSSAGDH	NGMLSVPAPG
2510	2520	2530	2540	2550
VPHSDVGEPP	ASTGAQQPSA	LAALQPAKER	RSSSSSSSSS	SSSSSSSSSS
2560	2570	2580	2590	2600
SSSSSSGSSS	SDSEGSSLPV	QPEVALKRVP	SPTPAPKEAV	REGRPPEPTP
2610	2620	2630	2640	2650
AKRKRRSSSS	SSSSSSSSSS	SSSSSSSSSS	SSSSSSSSSS	SSSSSSSSPS
2660	2670	2680	2690	2700
PAKPGPQALP	KPASPKKPPP	GERRSRSPRK	PIDSLRDSRS	LSYSPVERRR
2710	2720	2730	2740	2750
PSPQPSPRDQ	QSSSSERGSR	RGQRGDSRSP	SHKRRRETPS	PRPMRHRSSR
2752
SP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1008 Collapse assay details

Data source: Panorama

Official Gene Symbol: SRRM2
Peptide Modified Sequence: ENS[+80.0]FGSPLEFR
Modification Type: Phospho (ST)
Protein - Site of Modification: 1326
Peptide - Site of Modification: 3
Peptide Start: 1324
Peptide End: 1334

CPTAC ID: CPTAC-1008
Peptide Molecular Mass: 1,361.5653
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	49.3	15.9	7.2	34.6	16.4	13.9	60.2	22.8	15.7	14	23	15
y7 (1+)	38.7	14.4	18.2	24.3	16.2	19.2	45.7	21.7	26.5	14	23	15
y8 (1+)	76.1	24.9	54.1	68.6	29.4	80.4	102.5	38.5	96.9	14	23	15
sum	44.2	13.5	9.2	29.5	14.4	12	53.1	19.7	15.1	14	23	15

SRRM2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1008 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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SRRM2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1008 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments