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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TACC2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TACC2 N/A
Sequence Length (AA) Molecular Weight (Da)
2948 309427
Protein Name
Transforming acidic coiled-coil-containing protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGNENSTSDN QRTLSAQTPR SAQPPGNSQN IKRKQQDTPG SPDHRDASSI
60 70 80 90 100
GSVGLGGFCT ASESSASLDP CLVSPEVTEP RKDPQGARGP EGSLLPSPPP
110 120 130 140 150
SQEREHPSSS MPFAECPPEG CLASPAAAPE DGPQTQSPRR EPAPNAPGDI
160 170 180 190 200
AAAFPAERDS STPYQEIAAV PSAGRERQPK EEGQKSSFSF SSGIDQSPGM
210 220 230 240 250
SPVPLREPMK APLCGEGDQP GGFESQEKEA AGGFPPAESR QGVASVQVTP
260 270 280 290 300
EAPAAAQQGT ESSAVLEKSP LKPMAPIPQD PAPRASDRER GQGEAPPQYL
310 320 330 340 350
TDDLEFLRAC HLPRSNSGAA PEAEVNAASQ ESCQQPVGAY LPHAELPWGL
360 370 380 390 400
PSPALVPEAG GSGKEALDTI DVQGHPQTGM RGTKPNQVVC VAAGGQPEGG
410 420 430 440 450
LPVSPEPSLL TPTEEAHPAS SLASFPAAQI PIAVEEPGSS SRESVSKAGM
460 470 480 490 500
PVSADAAKEV VDAGLVGLER QVSDLGSKGE HPEGDPGEVP APSPQERGEH
510 520 530 540 550
LNTEQSHEVQ PGVPPPPLPK EQSHEVQPGA PPPPLPKAPS ESARGPPGPT
560 570 580 590 600
DGAKVHEDST SPAVAKEGSR SPGDSPGGKE EAPEPPDGGD PGNLQGEDSQ
610 620 630 640 650
AFSSKRDPEV GKDELSKPSS DAESRDHPSS HSAQPPRKGG AGHTDGPHSQ
660 670 680 690 700
TAEADASGLP HKLGEEDPVL PPVPDGAGEP TVPEGAIWEG SGLQPKCPDT
710 720 730 740 750
LQSREGLGRM ESFLTLESEK SDFPPTPVAE VAPKAQEGES TLEIRKMGSC
760 770 780 790 800
DGEGLLTSPD QPRGPACDAS RQEFHAGVPH PPQGENLAAD LGLTALILDQ
810 820 830 840 850
DQQGIPSCPG EGWIRGAASE WPLLSSEKHL QPSQAQPETS IFDVLKEQAQ
860 870 880 890 900
PPENGKETSP SHPGFKDQGA DSSQIHVPVE PQEDNNLPTH GGQEQALGSE
910 920 930 940 950
LQSQLPKGTL SDTPTSSPTD MVWESSLTEE SELSAPTRQK LPALGEKRPE
960 970 980 990 1000
GACGDGQSSR VSPPAADVLK DFSLAGNFSR KETCCTGQGP NKSQQALADA
1010 1020 1030 1040 1050
LEEGSQHEEA CQRHPGASEA ADGCSPLWGL SKREMASGNT GEAPPCQPDS
1060 1070 1080 1090 1100
VALLDAVPCL PALAPASPGV TPTQDAPETE ACDETQEGRQ QPVPAPQQKM
1110 1120 1130 1140 1150
ECWATSDAES PKLLASFPSA GEQGGEAGAA ETGGSAGAGD PGKQQAPEKP
1160 1170 1180 1190 1200
GEATLSCGLL QTEHCLTSGE EASTSALRES CQAEHPMASC QDALLPAREL
1210 1220 1230 1240 1250
GGIPRSTMDF STHQAVPDPK ELLLSGPPEV AAPDTPYLHV DSAAQRGAED
1260 1270 1280 1290 1300
SGVKAVSSAD PRAPGESPCP VGEPPLALEN AASLKLFAGS LAPLLQPGAA
1310 1320 1330 1340 1350
GGEIPAVQAS SGSPKARTTE GPVDSMPCLD RMPLLAKGKQ ATGEEKAATA
1360 1370 1380 1390 1400
PGAGAKASGE GMAGDAAGET EGSMERMGEP SQDPKQGTSG GVDTSSEQIA
1410 1420 1430 1440 1450
TLTGFPDFRE HIAKIFEKPV LGALATPGEK AGAGRSAVGK DLTRPLGPEK
1460 1470 1480 1490 1500
LLDGPPGVDV TLLPAPPARL QVEKKQQLAG EAEISHLALQ DPASDKLLGP
1510 1520 1530 1540 1550
AGLTWERNLP GAGVGKEMAG VPPTLREDER PEGPGAAWPG LEGQAYSQLE
1560 1570 1580 1590 1600
RSRQELASGL PSPAATQELP VERAAAFQVA PHSHGEEAVA QDRIPSGKQH
1610 1620 1630 1640 1650
QETSACDSPH GEDGPGDFAH TGVPGHVPRS TCAPSPQREV LTVPEANSEP
1660 1670 1680 1690 1700
WTLDTLGGER RPGVTAGILE MRNALGNQST PAPPTGEVAD TPLEPGKVAG
1710 1720 1730 1740 1750
AAGEAEGDIT LSTAETQACA SGDLPEAGTT RTFSVVAGDL VLPGSCQDPA
1760 1770 1780 1790 1800
CSDKAPGMEG TAALHGDSPA RPQQAKEQPG PERPIPAGDG KVCVSSPPEP
1810 1820 1830 1840 1850
DETHDPKLQH LAPEELHTDR ESPRPGPSML PSVPKKDAPR VMDKVTSDET
1860 1870 1880 1890 1900
RGAEGTESSP VADDIIQPAA PADLESPTLA ASSYHGDVVG QVSTDLIAQS
1910 1920 1930 1940 1950
ISPAAAHAGL PPSAAEHIVS PSAPAGDRVE ASTPSCPDPA KDLSRSSDSE
1960 1970 1980 1990 2000
EAFETPESTT PVKAPPAPPP PPPEVIPEPE VSTQPPPEEP GCGSETVPVP
2010 2020 2030 2040 2050
DGPRSDSVEG SPFRPPSHSF SAVFDEDKPI ASSGTYNLDF DNIELVDTFQ
2060 2070 2080 2090 2100
TLEPRASDAK NQEGKVNTRR KSTDSVPISK STLSRSLSLQ ASDFDGASSS
2110 2120 2130 2140 2150
GNPEAVALAP DAYSTGSSSA SSTLKRTKKP RPPSLKKKQT TKKPTETPPV
2160 2170 2180 2190 2200
KETQQEPDEE SLVPSGENLA SETKTESAKT EGPSPALLEE TPLEPAVGPK
2210 2220 2230 2240 2250
AACPLDSESA EGVVPPASGG GRVQNSPPVG RKTLPLTTAP EAGEVTPSDS
2260 2270 2280 2290 2300
GGQEDSPAKG LSVRLEFDYS EDKSSWDNQQ ENPPPTKKIG KKPVAKMPLR
2310 2320 2330 2340 2350
RPKMKKTPEK LDNTPASPPR SPAEPNDIPI AKGTYTFDID KWDDPNFNPF
2360 2370 2380 2390 2400
SSTSKMQESP KLPQQSYNFD PDTCDESVDP FKTSSKTPSS PSKSPASFEI
2410 2420 2430 2440 2450
PASAMEANGV DGDGLNKPAK KKKTPLKTDT FRVKKSPKRS PLSDPPSQDP
2460 2470 2480 2490 2500
TPAATPETPP VISAVVHATD EEKLAVTNQK WTCMTVDLEA DKQDYPQPSD
2510 2520 2530 2540 2550
LSTFVNETKF SSPTEELDYR NSYEIEYMEK IGSSLPQDDD APKKQALYLM
2560 2570 2580 2590 2600
FDTSQESPVK SSPVRMSESP TPCSGSSFEE TEALVNTAAK NQHPVPRGLA
2610 2620 2630 2640 2650
PNQESHLQVP EKSSQKELEA MGLGTPSEAI EITAPEGSFA SADALLSRLA
2660 2670 2680 2690 2700
HPVSLCGALD YLEPDLAEKN PPLFAQKLQE ELEFAIMRIE ALKLARQIAL
2710 2720 2730 2740 2750
ASRSHQDAKR EAAHPTDVSI SKTALYSRIG TAEVEKPAGL LFQQPDLDSA
2760 2770 2780 2790 2800
LQIARAEIIT KEREVSEWKD KYEESRREVM EMRKIVAEYE KTIAQMIEDE
2810 2820 2830 2840 2850
QREKSVSHQT VQQLVLEKEQ ALADLNSVEK SLADLFRRYE KMKEVLEGFR
2860 2870 2880 2890 2900
KNEEVLKRCA QEYLSRVKKE EQRYQALKVH AEEKLDRANA EIAQVRGKAQ
2910 2920 2930 2940 2948
QEQAAHQASL RKEQLRVDAL ERTLEQKNKE IEELTKICDE LIAKMGKS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1011 Collapse assay details

Data source: Panorama

Official Gene Symbol
TACC2
Peptide Modified Sequence
FSS[+80.0]PTEELDYR
Modification Type
Phospho (ST)
Protein - Site of Modification
2512
Peptide - Site of Modification
3
Peptide Start
2510
Peptide End
2520
CPTAC ID
CPTAC-1011
Peptide Molecular Mass
1,422.5704
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b9 (1+) 38.6 20.7 20.8 29.6 23.8 19.8 48.6 31.5 28.7 15 24 15
y9-98 (2+) 32.8 5.9 5.8 16.2 7.5 8.9 36.6 9.5 10.6 15 24 15
y8 (1+) 33.4 7.2 7.5 21.1 9 7.3 39.5 11.5 10.5 15 24 15
sum 32.6 6 5.5 16.4 7.7 7.1 36.5 9.8 9 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments