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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TNKS1BP1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TNKS1BP1 KIAA1741, TAB182
Sequence Length (AA) Molecular Weight (Da)
1729 181796
Protein Name
182 kDa tankyrase-1-binding protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKVSTLRESS AMASPLPREM EEELVPTGSE PGDTRAKPPV KPKPRALPAK
60 70 80 90 100
PALPAKPSLL VPVGPRPPRG PLAELPSARK MNMLAGPQPY GGSKRPLPFA
110 120 130 140 150
PRPAVEASTG GEATQETGKE EAGKEEPPPL TPPARCAAPG GVRKAPAPFR
160 170 180 190 200
PASERFAATT VEEILAKMEQ PRKEVLASPD RLWGSRLTFN HDGSSRYGPR
210 220 230 240 250
TYGTTTAPRD EDGSTLFRGW SQEGPVKSPA ECREEHSKTP EERSLPSDLA
260 270 280 290 300
FNGDLAKAAS SELPADISKP WIPSSPAPSS ENGGPASPGL PAEASGSGPG
310 320 330 340 350
SPHLHPPDKS SPCHSQLLEA QTPEASQASP CPAVTPSAPS AALPDEGSRH
360 370 380 390 400
TPSPGLPAEG APEAPRPSSP PPEVLEPHSL DQPPATSPRP LIEVGELLDL
410 420 430 440 450
TRTFPSGGEE EAKGDAHLRP TSLVQRRFSE GVLQSPSQDQ EKLGGSLAAL
460 470 480 490 500
PQGQGSQLAL DRPFGAESNW SLSQSFEWTF PTRPSGLGVW RLDSPPPSPI
510 520 530 540 550
TEASEAAEAA EAGNLAVSSR EEGVSQQGQG AGSAPSGSGS SWVQGDDPSM
560 570 580 590 600
SLTQKGDGES QPQFPAVPLE PLPTTEGTPG LPLQQAEERY ESQEPLAGQE
610 620 630 640 650
SPLPLATREA ALPILEPVLG QEQPAAPDQP CVLFADAPEP GQALPVEEEA
660 670 680 690 700
VTLARAETTQ ARTEAQDLCR ASPEPPGPES SSRWLDDLLA SPPPSGGGAR
710 720 730 740 750
RGAGAELKDT QSPSTCSEGL LGWSQKDLQS EFGITGDPQP SSFSPSSWCQ
760 770 780 790 800
GASQDYGLGG ASPRGDPGLG ERDWTSKYGQ GAGEGSTREW ASRCGIGQEE
810 820 830 840 850
MEASSSQDQS KVSAPGVLTA QDRVVGKPAQ LGTQRSQEAD VQDWEFRKRD
860 870 880 890 900
SQGTYSSRDA ELQDQEFGKR DSLGTYSSRD VSLGDWEFGK RDSLGAYASQ
910 920 930 940 950
DANEQGQDLG KRDHHGRYSS QDADEQDWEF QKRDVSLGTY GSRAAEPQEQ
960 970 980 990 1000
EFGKSAWIRD YSSGGSSRTL DAQDRSFGTR PLSSGFSPEE AQQQDEEFEK
1010 1020 1030 1040 1050
KIPSVEDSLG EGSRDAGRPG ERGSGGLFSP STAHVPDGAL GQRDQSSWQN
1060 1070 1080 1090 1100
SDASQEVGGH QERQQAGAQG PGSADLEDGE MGKRGWVGEF SLSVGPQREA
1110 1120 1130 1140 1150
AFSPGQQDWS RDFCIEASER SYQFGIIGND RVSGAGFSPS SKMEGGHFVP
1160 1170 1180 1190 1200
PGKTTAGSVD WTDQLGLRNL EVSSCVGSGG SSEARESAVG QMGWSGGLSL
1210 1220 1230 1240 1250
RDMNLTGCLE SGGSEEPGGI GVGEKDWTSD VNVKSKDLAE VGEGGGHSQA
1260 1270 1280 1290 1300
RESGVGQTDW SGVEAGEFLK SRERGVGQAD WTPDLGLRNM APGAVCSPGE
1310 1320 1330 1340 1350
SKELGVGQMD WGNNLGLRDL EVTCDPDSGG SQGLRGCGVG QMDWTQDLAP
1360 1370 1380 1390 1400
QNVELFGAPS EAREHGVGGV SQCPEPGLRH NGSLSPGLEA RDPLEARELG
1410 1420 1430 1440 1450
VGETSGPETQ GEDYSSSSLE PHPADPGMET GEALSFGASP GRCPARPPPS
1460 1470 1480 1490 1500
GSQGLLEEML AASSSKAVAR RESAASGLGG LLEEEGAGAG AAQEEVLEPG
1510 1520 1530 1540 1550
RDSPPSWRPQ PDGEASQTED VDGTWGSSAA RWSDQGPAQT SRRPSQGPPA
1560 1570 1580 1590 1600
RSPSQDFSFI EDTEILDSAM YRSRANLGRK RGHRAPVIRP GGTLGLSEAA
1610 1620 1630 1640 1650
DSDAHLFQDS TEPRASRVPS SDEEVVEEPQ SRRTRMSLGT KGLKVNLFPG
1660 1670 1680 1690 1700
LSPSALKAKL RPRNRSAEEG ELAESKSSQK ESAVQRSKSC KVPGLGKPLT
1710 1720 1729
LPPKPEKSSG SEGSSPNWLQ ALKLKKKKV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1013 Collapse assay details

Data source: Panorama

Official Gene Symbol
TNKS1BP1
Peptide Modified Sequence
S[+80.0]QEADVQDWEFR
Modification Type
Phospho (ST)
Protein - Site of Modification
836
Peptide - Site of Modification
1
Peptide Start
836
Peptide End
847
CPTAC ID
CPTAC-1013
Peptide Molecular Mass
1,588.6195
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 35.1 9.2 7.1 26.1 13.3 9.1 43.7 16.2 11.5 15 24 15
y8 (1+) 28.7 9.7 7.1 28.8 14.9 8.7 40.7 17.8 11.2 15 24 15
y9 (1+) 38.7 9.6 7.6 31.4 13.5 8.8 49.8 16.6 11.6 15 24 15
sum 31.8 8.8 6.9 22.6 13.3 8.5 39 15.9 10.9 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1012 Collapse assay details

Data source: Panorama

Official Gene Symbol
TNKS1BP1
Peptide Modified Sequence
YESQEPLAGQES[+80.0]PLPLATR
Modification Type
Phospho (ST)
Protein - Site of Modification
601
Peptide - Site of Modification
12
Peptide Start
590
Peptide End
608
CPTAC ID
CPTAC-1012
Peptide Molecular Mass
2,165.0042
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b8 (1+) 39 10.6 10.1 35.6 18.4 11.8 52.8 21.2 15.5 15 24 15
y11 (2+) 53.3 14 12.9 42.6 15.1 13.6 68.2 20.6 18.7 15 24 15
y14 (2+) 39.9 10.6 11.2 40.4 14.3 13.5 56.8 17.8 17.5 15 24 15
sum 38.4 10.3 9.8 28.5 14.1 11.5 47.8 17.5 15.1 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments