TPX2, CPTAC-1016 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TPX2	C20orf1, C20orf2, DIL2, HCA519

Sequence Length (AA)	Molecular Weight (Da)
747	85653

Protein Name
Targeting protein for Xklp2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSQVKSSYSY	DAPSDFINFS	SLDDEGDTQN	IDSWFEEKAN	LENKLLGKNG
60	70	80	90	100
TGGLFQGKTP	LRKANLQQAI	VTPLKPVDNT	YYKEAEKENL	VEQSIPSNAC
110	120	130	140	150
SSLEVEAAIS	RKTPAQPQRR	SLRLSAQKDL	EQKEKHHVKM	KAKRCATPVI
160	170	180	190	200
IDEILPSKKM	KVSNNKKKPE	EEGSAHQDTA	EKNASSPEKA	KGRHTVPCMP
210	220	230	240	250
PAKQKFLKST	EEQELEKSMK	MQQEVVEMRK	KNEEFKKLAL	AGIGQPVKKS
260	270	280	290	300
VSQVTKSVDF	HFRTDERIKQ	HPKNQEEYKE	VNFTSELRKH	PSSPARVTKG
310	320	330	340	350
CTIVKPFNLS	QGKKRTFDET	VSTYVPLAQQ	VEDFHKRTPN	RYHLRSKKDD
360	370	380	390	400
INLLPSKSSV	TKICRDPQTP	VLQTKHRARA	VTCKSTAELE	AEELEKLQQY
410	420	430	440	450
KFKARELDPR	ILEGGPILPK	KPPVKPPTEP	IGFDLEIEKR	IQERESKKKT
460	470	480	490	500
EDEHFEFHSR	PCPTKILEDV	VGVPEKKVLP	ITVPKSPAFA	LKNRIRMPTK
510	520	530	540	550
EDEEEDEPVV	IKAQPVPHYG	VPFKPQIPEA	RTVEICPFSF	DSRDKERQLQ
560	570	580	590	600
KEKKIKELQK	GEVPKFKALP	LPHFDTINLP	EKKVKNVTQI	EPFCLETDRR
610	620	630	640	650
GALKAQTWKH	QLEEELRQQK	EAACFKARPN	TVISQEPFVP	KKEKKSVAEG
660	670	680	690	700
LSGSLVQEPF	QLATEKRAKE	RQELEKRMAE	VEAQKAQQLE	EARLQEEEQK
710	720	730	740	747
KEELARLRRE	LVHKANPIRK	YQGLEIKSSD	QPLTVPVSPK	FSTRFHC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1016 Collapse assay details

Data source: Panorama

Official Gene Symbol: TPX2
Peptide Modified Sequence: SSDQPLTVPVS[+80.0]PK
Modification Type: Phospho (ST)
Protein - Site of Modification: 738
Peptide - Site of Modification: 11
Peptide Start: 728
Peptide End: 740

CPTAC ID: CPTAC-1016
Peptide Molecular Mass: 1,433.6803
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	24	8.2	11.6	20.8	9.3	11.6	31.8	12.4	16.4	15	24	15
y7 (1+)	20.1	11.3	16	18.8	11	28	27.5	15.8	32.2	15	24	15
y9 (1+)	25.4	6.8	7	20	7.2	8.7	32.3	9.9	11.2	15	24	15
sum	22.8	7.4	9.5	19.3	7.5	10.5	29.9	10.5	14.2	15	24	15

TPX2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1016 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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TPX2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1016 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments