LARP1B, CPTAC-1030 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
LARP1B	LARP2

Sequence Length (AA)	Molecular Weight (Da)
914	105322

Protein Name
La-related protein 1B

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MENWPTPSEL	VNTGFQSVLS	QGNKKPQNRK	EKEEKVEKRS	NSDSKENRET
60	70	80	90	100
KLNGPGENVS	EDEAQSSNQR	KRANKHKWVP	LHLDVVRSES	QERPGSRNSS
110	120	130	140	150
RCQPEANKPT	HNNRRNDTRS	WKRDREKRDD	QDDVSSVRSE	GGNIRGSFRG
160	170	180	190	200
RGRGRGRGRG	RGRGNPRLNF	DYSYGYQEHG	ERTDQPFQTE	LNTSMMYYYD
210	220	230	240	250
DGTGVQVYPV	EEALLKEYIK	RQIEYYFSVE	NLERDFFLRG	KMDEQGFLPI
260	270	280	290	300
SLIAGFQRVQ	ALTTNLNLIL	EALKDSTEVE	IVDEKMRKKI	EPEKWPIPGP
310	320	330	340	350
PPRSVPPTDF	SQLIDCPEFV	PGQAFCSHTE	SAPNSPRIGS	PLSPKKNSET
360	370	380	390	400
SILQAMSRGL	STSLPDLDSE	PWIEVKKRHQ	PAPVKLRESV	SVPEGSLNQL
410	420	430	440	450
CSSEEPEQEE	LDFLFDEEIE	QIGRKNTFTD	WSDNDSDYEI	DDQDLNKILI
460	470	480	490	500
VTQTPPYVKK	HPGGDRTGTH	MSRAKITSEL	AKVINDGLYY	YEQDLWMEED
510	520	530	540	550
ENKHTAIKQE	VENFKKLNLI	SKEQFENLTP	ELPFEPNQEV	PVAPSQSRQG
560	570	580	590	600
GVQGVLHIPK	KDLTDELAQK	LFDVSEITSA	AMVHSLPTAV	PESPRIHPTR
610	620	630	640	650
TPKTPRTPRL	QDPNKTPRFY	PVVKEPKAID	VKSPRKRKTR	HSTNPPLECH
660	670	680	690	700
VGWVMDSRDR	GPGTSSVSTS	NASPSEGAPL	AGSYGCTPHS	FPKFQHPSHE
710	720	730	740	750
LLKENGFTQQ	VYHKYRRRCL	SERKRLGIGQ	SQEMNTLFRF	WSFFLRDHFN
760	770	780	790	800
KKMYEEFRQL	AWEDAKENYR	YGLECLFRFY	SYGLEKKFRR	EIFQDFQEET
810	820	830	840	850
KKDYESGQLY	GLEKFWAYLK	YSQSKTQSID	PKLQEYLCSF	KRLEDFRVDP
860	870	880	890	900
PISDEFGRKR	HSSTSGEESN	RHRLPPNSST	KPPNAAKPTS	TSELQVPINS
910	914
PRRNISPESS	DNSH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1030 Collapse assay details

Data source: Panorama

Official Gene Symbol: LARP1B
Peptide Modified Sequence: GLSTS[+80.0]LPDLDSEPWIEVK
Modification Type: Phospho (ST)
Protein - Site of Modification: 363
Peptide - Site of Modification: 5
Peptide Start: 359
Peptide End: 376

CPTAC ID: CPTAC-1030
Peptide Molecular Mass: 2,064.9657
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	30.4	16.7	10.7	43	56.3	21.2	52.7	58.7	23.7	11	18	13
y8 (1+)	65.6	28.3	16	57.9	55.2	29.4	87.5	62	33.5	11	18	13
y9 (1+)	41	33.8	25.7	50.8	70.1	25	65.3	77.8	35.9	11	18	13
sum	20.5	19.1	9.8	20.9	56.5	21.3	29.3	59.6	23.4	11	18	13

LARP1B

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1030 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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LARP1B

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1030 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments