CASP10, CPTAC-1039 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CASP10	MCH4

Sequence Length (AA)	Molecular Weight (Da)
521	58951

Protein Name
Caspase-10

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKSQGQHWYS	SSDKNCKVSF	REKLLIIDSN	LGVQDVENLK	FLCIGLVPNK
60	70	80	90	100
KLEKSSSASD	VFEHLLAEDL	LSEEDPFFLA	ELLYIIRQKK	LLQHLNCTKE
110	120	130	140	150
EVERLLPTRQ	RVSLFRNLLY	ELSEGIDSEN	LKDMIFLLKD	SLPKTEMTSL
160	170	180	190	200
SFLAFLEKQG	KIDEDNLTCL	EDLCKTVVPK	LLRNIEKYKR	EKAIQIVTPP
210	220	230	240	250
VDKEAESYQG	EEELVSQTDV	KTFLEALPQE	SWQNKHAGSN	GNRATNGAPS
260	270	280	290	300
LVSRGMQGAS	ANTLNSETST	KRAAVYRMNR	NHRGLCVIVN	NHSFTSLKDR
310	320	330	340	350
QGTHKDAEIL	SHVFQWLGFT	VHIHNNVTKV	EMEMVLQKQK	CNPAHADGDC
360	370	380	390	400
FVFCILTHGR	FGAVYSSDEA	LIPIREIMSH	FTALQCPRLA	EKPKLFFIQA
410	420	430	440	450
CQGEEIQPSV	SIEADALNPE	QAPTSLQDSI	PAEADFLLGL	ATVPGYVSFR
460	470	480	490	500
HVEEGSWYIQ	SLCNHLKKLV	PRMLKFLEKT	MEIRGRKRTV	WGAKQISATS
510	520	521
LPTAISAQTP	RPPMRRWSSV	S

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1039 Collapse assay details

Data source: Panorama

Official Gene Symbol: CASP10
Peptide Sequence: LLIIDSNLGVQDVENLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 24
Peptide End: 40

CPTAC ID: CPTAC-1039
Peptide Molecular Mass: 1,882.0411
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	37.4	19.9	21	69.9	25.9	33.8	79.3	32.7	39.8	14	15	15
y7 (1+)	46	50.8	32.1	75.4	51	36.9	88.3	72	48.9	15	14	15
y4 (1+)	66.3	30.9	56.3	85.2	44.5	53.8	108	54.2	77.9	15	15	15
sum	33.4	16.3	15.6	50	22	25.3	60.1	27.4	29.7	15	15	15

CASP10

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1039 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CASP10

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1039 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments