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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PRDX3 AOP1
Sequence Length (AA) Molecular Weight (Da)
256 27693
Protein Name
Thioredoxin-dependent peroxide reductase, mitochondrial
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAAVGRLLR ASVARHVSAI PWGISATAAL RPAACGRTSL TNLLCSGSSQ
60 70 80 90 100
AKLFSTSSSC HAPAVTQHAP YFKGTAVVNG EFKDLSLDDF KGKYLVLFFY
110 120 130 140 150
PLDFTFVCPT EIVAFSDKAN EFHDVNCEVV AVSVDSHFSH LAWINTPRKN
160 170 180 190 200
GGLGHMNIAL LSDLTKQISR DYGVLLEGSG LALRGLFIID PNGVIKHLSV
210 220 230 240 250
NDLPVGRSVE ETLRLVKAFQ YVETHGEVCP ANWTPDSPTI KPSPAASKEY
256
FQKVNQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-115 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRDX3
Peptide Sequence
GLFIIDPNGVIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
185
Peptide End
196
CPTAC ID
CPTAC-115
Peptide Molecular Mass
1,284.7442
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 6.7 6.2 3.6 12.3 8.6 7.9 14 10.6 8.7 15 25 15
y8 (1+) 6.5 6.9 3.8 17.7 8.6 11.3 18.9 11 11.9 15 25 15
y7 (1+) 9.2 7 5.1 20.8 9.5 9.5 22.7 11.8 10.8 15 25 15
sum 6 6.6 4 17.6 8.7 9.9 18.6 10.9 10.7 15 25 15

Selectivity

Data source: Panorama

Slope of Curve Fit for cell line
fragment_ion HCC1187 HCC1419 HCC1954 HCC70 MCF12A MDAMB175VII ZR751 ZR7530
y7 (1+) -0.26 -0.06 -0.17 -0.28 -0.05 -0.02 -0.06 -0.05
y9 (1+) -0.27 -0.07 -0.15 -0.24 -0.07 -0.08 -0.07 -0.01
y8 (1+) -0.27 -0.06 -0.16 -0.28 -0.05 -0.03 -0.08 -0.03
sum -0.27 -0.06 -0.16 -0.27 -0.05 -0.03 -0.07 -0.04

Stability

Data source: Panorama

Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y7 (1+) 1.9 1.2 2.5 NA 3.6
y8 (1+) 2.5 2.3 2.4 NA 2
y9 (1+) 3.2 5.5 2.5 NA 7
sum 2.1 1.7 1.6 NA 2
Fragment ion / Transition all_intra_CV all_inter_CV
y7 (1+) 2.1 2.6
y8 (1+) 2.3 2
y9 (1+) 4.7 4.4
sum 1.8 1.7

Endogenous

Data source: Panorama

Fragment ion / Transition intra_CV inter_CV total_CV
y7 (1+) 7.2 9.5 11.9
y9 (1+) 6.4 8.3 10.5
y8 (1+) 7 8.7 11.2
sum 6.8 8.7 11

Additional Resources and Comments


Assay Details for CPTAC-116 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRDX3
Peptide Sequence
HLSVNDLPVGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
197
Peptide End
207
CPTAC ID
CPTAC-116
Peptide Molecular Mass
1,205.6517
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 7.9 6.4 5.1 8.1 8 4.4 11.3 10.2 6.7 15 25 15
y8 (1+) 37 7.6 4.8 38 8.2 6.4 53 11.2 8 15 25 15
y7 (1+) 50.4 10.7 7.6 50.4 11.1 8.1 71.3 15.4 11.1 15 25 15
sum 8.4 6.2 5.2 8.6 7.1 3.7 12 9.4 6.4 15 25 15


Stability

Data source: Panorama

Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
Fragment ion / Transition all_intra_CV all_inter_CV

Additional Resources and Comments