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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

RPN1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
RPN1 N/A
Sequence Length (AA) Molecular Weight (Da)
607 68569
Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEAPAAGLFL LLLLGTWAPA PGSASSEAPP LINEDVKRTV DLSSHLAKVT
60 70 80 90 100
AEVVLAHLGG GSTSRATSFL LALEPELEAR LAHLGVQVKG EDEEENNLEV
110 120 130 140 150
RETKIKGKSG RFFTVKLPVA LDPGAKISVI VETVYTHVLH PYPTQITQSE
160 170 180 190 200
KQFVVFEGNH YFYSPYPTKT QTMRVKLASR NVESYTKLGN PTRSEDLLDY
210 220 230 240 250
GPFRDVPAYS QDTFKVHYEN NSPFLTITSM TRVIEVSHWG NIAVEENVDL
260 270 280 290 300
KHTGAVLKGP FSRYDYQRQP DSGISSIRSF KTILPAAAQD VYYRDEIGNV
310 320 330 340 350
STSHLLILDD SVEMEIRPRF PLFGGWKTHY IVGYNLPSYE YLYNLGDQYA
360 370 380 390 400
LKMRFVDHVF DEQVIDSLTV KIILPEGAKN IEIDSPYEIS RAPDELHYTY
410 420 430 440 450
LDTFGRPVIV AYKKNLVEQH IQDIVVHYTF NKVLMLQEPL LVVAAFYILF
460 470 480 490 500
FTVIIYVRLD FSITKDPAAE ARMKVACITE QVLTLVNKRI GLYRHFDETV
510 520 530 540 550
NRYKQSRDIS TLNSGKKSLE TEHKALTSEI ALLQSRLKTE GSDLCDRVSE
560 570 580 590 600
MQKLDAQVKE LVLKSAVEAE RLVAGKLKKD TYIENEKLIS GKRQELVTKI
607
DHILDAL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-126 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPN1
Peptide Sequence
NIEIDSPYEISR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
380
Peptide End
391
CPTAC ID
CPTAC-126
Peptide Molecular Mass
1,434.6991
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 15.6 5.7 7 14.9 11 9.2 21.6 12.4 11.6 15 25 15
y8 (1+) 24.2 9.2 6.8 24.1 11.1 6.7 34.2 14.4 9.5 15 25 15
y7 (1+) 17.9 7.4 7 25.7 11.1 9.2 31.3 13.3 11.6 15 25 15
sum 10.4 5.8 4.4 13.5 10 5.7 17 11.6 7.2 15 25 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-125 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPN1
Peptide Sequence
SEDLLDYGPFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
194
Peptide End
204
CPTAC ID
CPTAC-125
Peptide Molecular Mass
1,310.6143
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 21.9 7.4 6.7 21 9.8 7.3 30.3 12.3 9.9 15 25 15
y7 (1+) 16.5 5.2 3.6 23.4 7.7 6.4 28.6 9.3 7.3 15 25 15
y6 (1+) 20.8 6.4 4.4 22.4 8.2 6.3 30.6 10.4 7.7 15 25 15
sum 12.6 5.5 3.6 17.2 8 5.8 21.3 9.7 6.8 15 25 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments