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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SCARB2 CD36L2,LIMP2,LIMPII
Sequence Length (AA) Molecular Weight (Da)
478 54290
Protein Name
Lysosome membrane protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGRCCFYTAG TLSLLLLVTS VTLLVARVFQ KAVDQSIEKK IVLRNGTEAF
60 70 80 90 100
DSWEKPPLPV YTQFYFFNVT NPEEILRGET PRVEEVGPYT YRELRNKANI
110 120 130 140 150
QFGDNGTTIS AVSNKAYVFE RDQSVGDPKI DLIRTLNIPV LTVIEWSQVH
160 170 180 190 200
FLREIIEAML KAYQQKLFVT HTVDELLWGY KDEILSLIHV FRPDISPYFG
210 220 230 240 250
LFYEKNGTND GDYVFLTGED SYLNFTKIVE WNGKTSLDWW ITDKCNMING
260 270 280 290 300
TDGDSFHPLI TKDEVLYVFP SDFCRSVYIT FSDYESVQGL PAFRYKVPAE
310 320 330 340 350
ILANTSDNAG FCIPEGNCLG SGVLNVSICK NGAPIIMSFP HFYQADERFV
360 370 380 390 400
SAIEGMHPNQ EDHETFVDIN PLTGIILKAA KRFQINIYVK KLDDFVETGD
410 420 430 440 450
IRTMVFPVMY LNESVHIDKE TASRLKSMIN TTLIITNIPY IIMALGVFFG
460 470 478
LVFTWLACKG QGSMDEGTAD ERAPLIRT

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-128 Collapse assay details

Data source: Panorama

Official Gene Symbol
SCARB2
Peptide Modified Sequence
DEVLYVFPSDFC[+57.0]R
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
274
Peptide - Site of Modification
12
Peptide Start
263
Peptide End
275
CPTAC ID
CPTAC-128
Peptide Molecular Mass
1,645.7446
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 23.3 26.9 32.4 26.2 47.2 22.6 35.1 54.3 39.5 12 12 12
y8 (1+) 28.6 27.3 29.3 38 41 29.9 47.6 49.3 41.9 12 12 12
y7 (1+) 25.8 46 15.9 28.7 27.6 23.1 38.6 53.6 28 12 12 12
sum 17 22.1 12 17.1 22.1 16.7 24.1 31.3 20.6 12 12 12

Additional Resources and Comments


Assay Details for CPTAC-127 Collapse assay details

Data source: Panorama

Official Gene Symbol
SCARB2
Peptide Sequence
VEEVGPYTYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
83
Peptide End
92
CPTAC ID
CPTAC-127
Peptide Molecular Mass
1,211.5823
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 24.3 9.9 9.7 34.4 11.7 10.4 42.1 15.3 14.2 15 25 15
y7 (1+) 29.6 6.1 4.4 31.2 7.9 8.4 43 10 9.5 15 25 15
y6 (1+) 9 4.8 3.9 14.8 7.5 4.6 17.3 8.9 6 15 25 15
sum 11.5 4.9 4.1 16.4 7.1 4.8 20 8.6 6.3 15 25 15

Additional Resources and Comments