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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPINB1 ELANH2, MNEI, PI2
Sequence Length (AA) Molecular Weight (Da)
379 42742
Protein Name
Leukocyte elastase inhibitor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEQLSSANTR FALDLFLALS ENNPAGNIFI SPFSISSAMA MVFLGTRGNT
60 70 80 90 100
AAQLSKTFHF NTVEEVHSRF QSLNADINKR GASYILKLAN RLYGEKTYNF
110 120 130 140 150
LPEFLVSTQK TYGADLASVD FQHASEDARK TINQWVKGQT EGKIPELLAS
160 170 180 190 200
GMVDNMTKLV LVNAIYFKGN WKDKFMKEAT TNAPFRLNKK DRKTVKMMYQ
210 220 230 240 250
KKKFAYGYIE DLKCRVLELP YQGEELSMVI LLPDDIEDES TGLKKIEEQL
260 270 280 290 300
TLEKLHEWTK PENLDFIEVN VSLPRFKLEE SYTLNSDLAR LGVQDLFNSS
310 320 330 340 350
KADLSGMSGA RDIFISKIVH KSFVEVNEEG TEAAAATAGI ATFCMLMPEE
360 370 379
NFTADHPFLF FIRHNSSGSI LFLGRFSSP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-132 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINB1
Peptide Sequence
LGVQDLFNSSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
291
Peptide End
301
CPTAC ID
CPTAC-132
Peptide Molecular Mass
1,206.6245
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 13.9 7.8 6.5 13.9 7.3 7 19.7 10.7 9.6 15 25 15
y7 (1+) 8.9 7.2 8.8 8.5 8.6 9 12.3 11.2 12.6 15 25 15
y6 (1+) 16.5 14.4 9.5 18.5 13.9 11.2 24.8 20 14.7 15 25 15
sum 8.6 6.5 5.7 10.2 7.5 5 13.3 9.9 7.6 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-131 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINB1
Peptide Sequence
TYNFLPEFLVSTQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
97
Peptide End
110
CPTAC ID
CPTAC-131
Peptide Molecular Mass
1,685.8665
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 14.2 8 6.1 19.7 12.3 26.5 24.3 14.7 27.2 15 25 15
y9 (1+) 8.7 5.6 4.8 23.1 13 15.3 24.7 14.2 16 15 25 15
y12 (2+) 79.1 101.5 59.4 106.8 123.3 74 132.9 159.7 94.9 15 25 15
sum 10.8 5.7 4.3 22.5 12.6 15.6 25 13.8 16.2 15 25 15

Additional Resources and Comments