GRB7, CPTAC-149 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GRB7	N/A

Sequence Length (AA)	Molecular Weight (Da)
532	59681

Protein Name
Growth factor receptor-bound protein 7

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MELDLSPPHL	SSSPEDLCPA	PGTPPGTPRP	PDTPLPEEVK	RSQPLLIPTT
60	70	80	90	100
GRKLREEERR	ATSLPSIPNP	FPELCSPPSQ	SPILGGPSSA	RGLLPRDASR
110	120	130	140	150
PHVVKVYSED	GACRSVEVAA	GATARHVCEM	LVQRAHALSD	ETWGLVECHP
160	170	180	190	200
HLALERGLED	HESVVEVQAA	WPVGGDSRFV	FRKNFAKYEL	FKSSPHSLFP
210	220	230	240	250
EKMVSSCLDA	HTGISHEDLI	QNFLNAGSFP	EIQGFLQLRG	SGRKLWKRFF
260	270	280	290	300
CFLRRSGLYY	STKGTSKDPR	HLQYVADVNE	SNVYVVTQGR	KLYGMPTDFG
310	320	330	340	350
FCVKPNKLRN	GHKGLRIFCS	EDEQSRTCWL	AAFRLFKYGV	QLYKNYQQAQ
360	370	380	390	400
SRHLHPSCLG	SPPLRSASDN	TLVAMDFSGH	AGRVIENPRE	ALSVALEEAQ
410	420	430	440	450
AWRKKTNHRL	SLPMPASGTS	LSAAIHRTQL	WFHGRISREE	SQRLIGQQGL
460	470	480	490	500
VDGLFLVRES	QRNPQGFVLS	LCHLQKVKHY	LILPSEEEGR	LYFSMDDGQT
510	520	530	532
RFTDLLQLVE	FHQLNRGILP	CLLRHCCTRV	AL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-149 Collapse assay details

Data source: Panorama

Official Gene Symbol: GRB7
Peptide Sequence: FTDLLQLVEFHQLNR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 502
Peptide End: 516

CPTAC ID: CPTAC-149
Peptide Molecular Mass: 1,871.9894
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y13 (2+)	11.4	7.6	9.5	20.6	30.7	15.6	23.5	31.6	18.3	15	25	15
y11 (2+)	10.9	9.5	9.1	18.2	30.5	17.1	21.2	31.9	19.4	15	25	15
y10 (2+)	11	8.9	9.7	19.5	31.1	17.3	22.4	32.3	19.8	15	25	15
sum	9.2	6.5	8.7	18.5	30.7	16.2	20.7	31.4	18.4	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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