TPM4, CPTAC-155 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TPM4	N/A

Sequence Length (AA)	Molecular Weight (Da)
248	28522

Protein Name
Tropomyosin alpha-4 chain

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGLNSLEAV	KRKIQALQQQ	ADEAEDRAQG	LQRELDGERE	RREKAEGDVA
60	70	80	90	100
ALNRRIQLVE	EELDRAQERL	ATALQKLEEA	EKAADESERG	MKVIENRAMK
110	120	130	140	150
DEEKMEIQEM	QLKEAKHIAE	EADRKYEEVA	RKLVILEGEL	ERAEERAEVS
160	170	180	190	200
ELKCGDLEEE	LKNVTNNLKS	LEAASEKYSE	KEDKYEEEIK	LLSDKLKEAE
210	220	230	240	248
TRAEFAERTV	AKLEKTIDDL	EEKLAQAKEE	NVGLHQTLDQ	TLNELNCI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-155 Collapse assay details

Data source: Panorama

Official Gene Symbol: TPM4
Peptide Sequence: AEGDVAALNR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 45
Peptide End: 54

CPTAC ID: CPTAC-155
Peptide Molecular Mass: 1,014.5094
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	38.6	21.5	8.6	48.3	20.7	19.8	61.8	29.8	21.6	15	25	15
y6 (1+)	38.6	28	25.6	40.5	29.4	45.1	55.9	40.6	51.9	15	25	15
y5 (1+)	17.8	11.5	24.6	37.6	18.8	24	41.6	22	34.4	15	25	15
sum	30.2	18.7	6.7	41	19.1	17.2	50.9	26.7	18.5	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

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