ABCD3, CPTAC-157 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ABCD3	PMP70, PXMP1

Sequence Length (AA)	Molecular Weight (Da)
659	75476

Protein Name
ATP-binding cassette sub-family D member 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAFSKYLTA	RNSSLAGAAF	LLLCLLHKRR	RALGLHGKKS	GKPPLQNNEK
60	70	80	90	100
EGKKERAVVD	KVFFSRLIQI	LKIMVPRTFC	KETGYLVLIA	VMLVSRTYCD
110	120	130	140	150
VWMIQNGTLI	ESGIIGRSRK	DFKRYLLNFI	AAMPLISLVN	NFLKYGLNEL
160	170	180	190	200
KLCFRVRLTK	YLYEEYLQAF	TYYKMGNLDN	RIANPDQLLT	QDVEKFCNSV
210	220	230	240	250
VDLYSNLSKP	FLDIVLYIFK	LTSAIGAQGP	ASMMAYLVVS	GLFLTRLRRP
260	270	280	290	300
IGKMTITEQK	YEGEYRYVNS	RLITNSEEIA	FYNGNKREKQ	TVHSVFRKLV
310	320	330	340	350
EHLHNFILFR	FSMGFIDSII	AKYLATVVGY	LVVSRPFLDL	SHPRHLKSTH
360	370	380	390	400
SELLEDYYQS	GRMLLRMSQA	LGRIVLAGRE	MTRLAGFTAR	ITELMQVLKD
410	420	430	440	450
LNHGKYERTM	VSQQEKGIEG	VQVIPLIPGA	GEIIIADNII	KFDHVPLATP
460	470	480	490	500
NGDVLIRDLN	FEVRSGANVL	ICGPNGCGKS	SLFRVLGELW	PLFGGRLTKP
510	520	530	540	550
ERGKLFYVPQ	RPYMTLGTLR	DQVIYPDGRE	DQKRKGISDL	VLKEYLDNVQ
560	570	580	590	600
LGHILEREGG	WDSVQDWMDV	LSGGEKQRMA	MARLFYHKPQ	FAILDECTSA
610	620	630	640	650
VSVDVEGYIY	SHCRKVGITL	FTVSHRKSLW	KHHEYYLHMD	GRGNYEFKQI
659
TEDTVEFGS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-157 Collapse assay details

Data source: Panorama

Official Gene Symbol: ABCD3
Peptide Sequence: VLGELWPLFGGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 485
Peptide End: 496

CPTAC ID: CPTAC-157
Peptide Molecular Mass: 1,342.7398
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	12.7	8.1	6.6	27.7	15.4	11.3	30.5	17.4	13.1	15	25	15
y8 (1+)	10.8	8.7	5.7	21.7	15.2	11.2	24.2	17.5	12.6	15	25	15
y7 (1+)	8.9	7.4	5.6	18.7	14.6	11.2	20.7	16.4	12.5	15	25	15
sum	8.8	7.6	5.8	21.6	14.9	11.1	23.3	16.7	12.5	15	25	15

ABCD3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-157 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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ABCD3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-157 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments