Overview Data source: UniProt

Official Gene Symbol Other Aliases
CAPS N/A
Sequence Length (AA) Molecular Weight (Da)
189 20967
Protein Name
Calcyphosin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDAVDATMEK LRAQCLSRGA SGIQGLARFF RQLDRDGSRS LDADEFRQGL
60 70 80 90 100
AKLGLVLDQA EAEGVCRKWD RNGSGTLDLE EFLRALRPPM SQAREAVIAA
110 120 130 140 150
AFAKLDRSGD GVVTVDDLRG VYSGRAHPKV RSGEWTEDEV LRRFLDNFDS
160 170 180 189
SEKDGQVTLA EFQDYYSGVS ASMNTDEEFV AMMTSAWQL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



loading

Loader

Assay Details for CPTAC-179 Collapse assay details

Data source: Panorama

Official Gene Symbol
CAPS
Peptide Sequence
EAVIAAAFAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
95
Peptide End
104
CPTAC ID
CPTAC-179
Peptide Molecular Mass
989.5546
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 15.3 18.9 32.1 39.2 35.9 32.3 42.1 40.6 45.5 15 25 15
y6 (1+) 5.3 6.5 7 7.4 8.7 8.3 9.1 10.9 10.9 15 25 15
y5 (1+) 6 9.5 5.3 11.7 10.7 5.7 13.1 14.3 7.8 15 25 15
sum 5.3 8.5 10.3 14.3 13.2 11.6 15.3 15.7 15.5 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-180 Collapse assay details

Data source: Panorama

Official Gene Symbol
CAPS
Peptide Sequence
SGDGVVTVDDLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
108
Peptide End
119
CPTAC ID
CPTAC-180
Peptide Molecular Mass
1,231.6044
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 14.7 9.9 12 15.9 10.3 12.5 21.7 14.3 17.3 15 25 15
y7 (1+) 4.2 5.3 3.8 4.5 6.4 3.6 6.2 8.3 5.2 15 25 15
y6 (1+) 5.9 5.1 3.5 6.1 6.3 2.9 8.5 8.1 4.5 15 25 15
sum 4.7 5 3.8 5 6.1 3.4 6.9 7.9 5.1 15 25 15

Additional Resources and Comments