ERP29, CPTAC-197 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ERP29	C12orf8, ERP28

Sequence Length (AA)	Molecular Weight (Da)
261	28993

Protein Name
Endoplasmic reticulum resident protein 29

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAAVPRAAF	LSPLLPLLLG	FLLLSAPHGG	SGLHTKGALP	LDTVTFYKVI
60	70	80	90	100
PKSKFVLVKF	DTQYPYGEKQ	DEFKRLAENS	ASSDDLLVAE	VGISDYGDKL
110	120	130	140	150
NMELSEKYKL	DKESYPVFYL	FRDGDFENPV	PYTGAVKVGA	IQRWLKGQGV
160	170	180	190	200
YLGMPGCLPV	YDALAGEFIR	ASGVEARQAL	LKQGQDNLSS	VKETQKKWAE
210	220	230	240	250
QYLKIMGKIL	DQGEDFPASE	MTRIARLIEK	NKMSDGKKEE	LQKSLNILTA
260	261
FQKKGAEKEE	L

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-197 Collapse assay details

Data source: Panorama

Official Gene Symbol: ERP29
Peptide Sequence: DGDFENPVPYTGAVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 123
Peptide End: 137

CPTAC ID: CPTAC-197
Peptide Molecular Mass: 1,607.7468
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	7.5	9.8	7.6	10	10.7	7.8	12.5	14.5	10.9	15	25	15
y10 (1+)	9.2	7.7	4.7	10	8	4.7	13.6	11.1	6.6	15	25	15
y9 (1+)	5.4	6.4	4.5	7.7	8.5	5	9.4	10.6	6.7	15	25	15
sum	5	6.2	4.3	7.5	8.1	4.7	9	10.2	6.4	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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