FKBP4, CPTAC-203 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FKBP4	FKBP52

Sequence Length (AA)	Molecular Weight (Da)
459	51805

Protein Name
Peptidyl-prolyl cis-trans isomerase FKBP4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTAEEMKATE	SGAQSAPLPM	EGVDISPKQD	EGVLKVIKRE	GTGTEMPMIG
60	70	80	90	100
DRVFVHYTGW	LLDGTKFDSS	LDRKDKFSFD	LGKGEVIKAW	DIAIATMKVG
110	120	130	140	150
EVCHITCKPE	YAYGSAGSPP	KIPPNATLVF	EVELFEFKGE	DLTEEEDGGI
160	170	180	190	200
IRRIQTRGEG	YAKPNEGAIV	EVALEGYYKD	KLFDQRELRF	EIGEGENLDL
210	220	230	240	250
PYGLERAIQR	MEKGEHSIVY	LKPSYAFGSV	GKEKFQIPPN	AELKYELHLK
260	270	280	290	300
SFEKAKESWE	MNSEEKLEQS	TIVKERGTVY	FKEGKYKQAL	LQYKKIVSWL
310	320	330	340	350
EYESSFSNEE	AQKAQALRLA	SHLNLAMCHL	KLQAFSAAIE	SCNKALELDS
360	370	380	390	400
NNEKGLFRRG	EAHLAVNDFE	LARADFQKVL	QLYPNNKAAK	TQLAVCQQRI
410	420	430	440	450
RRQLAREKKL	YANMFERLAE	EENKAKAEAS	SGDHPTDTEM	KEEQKSNTAG
459
SQSQVETEA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-202 Collapse assay details

Data source: Panorama

Official Gene Symbol: FKBP4
Peptide Sequence: VFVHYTGWLLDGTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 53
Peptide End: 66

CPTAC ID: CPTAC-202
Peptide Molecular Mass: 1,634.8457
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (2+)	17.3	15.3	13.2	17.8	25.5	13.2	24.8	29.7	18.7	25	15	15
y8 (1+)	27.8	17	16.3	33.4	22.6	23.5	43.5	28.3	28.6	25	15	15
y10 (1+)	19	16.1	17.2	20.4	23	18.2	27.9	28.1	25	25	15	15
sum	17.3	15	14.2	20	23.1	15.5	26.4	27.5	21	25	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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