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Overview Data source: UniProt

Official Gene Symbol Other Aliases
GLRX3 PICOT, TXNL2
Sequence Length (AA) Molecular Weight (Da)
335 37432
Protein Name
Glutaredoxin-3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAGAAEAAV AAVEEVGSAG QFEELLRLKA KSLLVVHFWA PWAPQCAQMN
60 70 80 90 100
EVMAELAKEL PQVSFVKLEA EGVPEVSEKY EISSVPTFLF FKNSQKIDRL
110 120 130 140 150
DGAHAPELTK KVQRHASSGS FLPSANEHLK EDLNLRLKKL THAAPCMLFM
160 170 180 190 200
KGTPQEPRCG FSKQMVEILH KHNIQFSSFD IFSDEEVRQG LKAYSSWPTY
210 220 230 240 250
PQLYVSGELI GGLDIIKELE ASEELDTICP KAPKLEERLK VLTNKASVML
260 270 280 290 300
FMKGNKQEAK CGFSKQILEI LNSTGVEYET FDILEDEEVR QGLKAYSNWP
310 320 330 335
TYPQLYVKGE LVGGLDIVKE LKENGELLPI LRGEN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-207 Collapse assay details

Data source: Panorama

Official Gene Symbol
GLRX3
Peptide Modified Sequence
ELEASEELDTIC[+57.0]PK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
229
Peptide - Site of Modification
12
Peptide Start
218
Peptide End
231
CPTAC ID
CPTAC-207
Peptide Molecular Mass
1,632.7553
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 10.9 9.6 13.1 15 14.3 18.5 18.5 17.2 22.7 15 25 15
y8 (1+) 15.8 9.2 8.7 22.2 11.4 16.7 27.2 14.6 18.8 15 25 15
y7 (1+) 15.7 10.8 14.6 23.9 15.3 23.5 28.6 18.7 27.7 15 25 15
sum 7.9 7.9 10.9 17.7 12.7 18.3 19.4 15 21.3 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-206 Collapse assay details

Data source: Panorama

Official Gene Symbol
GLRX3
Peptide Sequence
LEAEGVPEVSEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
68
Peptide End
79
CPTAC ID
CPTAC-206
Peptide Molecular Mass
1,285.6402
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 6.4 6.4 6.6 14.1 15 18.1 15.5 16.3 19.3 15 25 15
y9 (1+) 9.7 7 8.6 16.6 17.7 18.2 19.2 19 20.1 15 25 15
y8 (1+) 4.6 7 6.6 10.1 15.5 16.3 11.1 17 17.6 15 25 15
sum 4.5 5 6.6 11.5 14.9 17.1 12.3 15.7 18.3 15 25 15

Additional Resources and Comments