GLRX3, CPTAC-207 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GLRX3	PICOT, TXNL2

Sequence Length (AA)	Molecular Weight (Da)
335	37432

Protein Name
Glutaredoxin-3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAGAAEAAV	AAVEEVGSAG	QFEELLRLKA	KSLLVVHFWA	PWAPQCAQMN
60	70	80	90	100
EVMAELAKEL	PQVSFVKLEA	EGVPEVSEKY	EISSVPTFLF	FKNSQKIDRL
110	120	130	140	150
DGAHAPELTK	KVQRHASSGS	FLPSANEHLK	EDLNLRLKKL	THAAPCMLFM
160	170	180	190	200
KGTPQEPRCG	FSKQMVEILH	KHNIQFSSFD	IFSDEEVRQG	LKAYSSWPTY
210	220	230	240	250
PQLYVSGELI	GGLDIIKELE	ASEELDTICP	KAPKLEERLK	VLTNKASVML
260	270	280	290	300
FMKGNKQEAK	CGFSKQILEI	LNSTGVEYET	FDILEDEEVR	QGLKAYSNWP
310	320	330	335
TYPQLYVKGE	LVGGLDIVKE	LKENGELLPI	LRGEN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-207 Collapse assay details

Data source: Panorama

Official Gene Symbol: GLRX3
Peptide Modified Sequence: ELEASEELDTIC[+57.0]PK
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: 229
Peptide - Site of Modification: 12
Peptide Start: 218
Peptide End: 231

CPTAC ID: CPTAC-207
Peptide Molecular Mass: 1,632.7553
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	10.9	9.6	13.1	15	14.3	18.5	18.5	17.2	22.7	15	25	15
y8 (1+)	15.8	9.2	8.7	22.2	11.4	16.7	27.2	14.6	18.8	15	25	15
y7 (1+)	15.7	10.8	14.6	23.9	15.3	23.5	28.6	18.7	27.7	15	25	15
sum	7.9	7.9	10.9	17.7	12.7	18.3	19.4	15	21.3	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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