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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ILF2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ILF2 NF45
Sequence Length (AA) Molecular Weight (Da)
390 43062
Protein Name
Interleukin enhancer-binding factor 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRGDRGRGRG GRFGSRGGPG GGFRPFVPHI PFDFYLCEMA FPRVKPAPDE
60 70 80 90 100
TSFSEALLKR NQDLAPNSAE QASILSLVTK INNVIDNLIV APGTFEVQIE
110 120 130 140 150
EVRQVGSYKK GTMTTGHNVA DLVVILKILP TLEAVAALGN KVVESLRAQD
160 170 180 190 200
PSEVLTMLTN ETGFEISSSD ATVKILITTV PPNLRKLDPE LHLDIKVLQS
210 220 230 240 250
ALAAIRHARW FEENASQSTV KVLIRLLKDL RIRFPGFEPL TPWILDLLGH
260 270 280 290 300
YAVMNNPTRQ PLALNVAYRR CLQILAAGLF LPGSVGITDP CESGNFRVHT
310 320 330 340 350
VMTLEQQDMV CYTAQTLVRI LSHGGFRKIL GQEGDASYLA SEISTWDGVI
360 370 380 390
VTPSEKAYEK PPEKKEGEEE EENTEEPPQG EEEESMETQE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-223 Collapse assay details

Data source: Panorama

Official Gene Symbol
ILF2
Peptide Sequence
ILPTLEAVAALGNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
128
Peptide End
141
CPTAC ID
CPTAC-223
Peptide Molecular Mass
1,408.8290
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 6.5 9.3 5.5 17.2 15.6 10.6 18.4 18.2 11.9 15 25 15
y9 (1+) 14.1 7.1 5.1 21.5 12.8 8.7 25.7 14.6 10.1 15 25 15
y8 (1+) 20.5 7.6 8.3 25.8 11 10.8 33 13.4 13.6 15 25 15
sum 9.1 6.9 6 18.4 12.8 9.7 20.5 14.5 11.4 15 25 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-224 Collapse assay details

Data source: Panorama

Official Gene Symbol
ILF2
Peptide Sequence
VLQSALAAIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
197
Peptide End
206
CPTAC ID
CPTAC-224
Peptide Molecular Mass
1,040.6342
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 11.7 10.1 10.2 11.5 21.8 14.5 16.4 24 17.7 15 25 15
y7 (1+) 6.1 6.6 6 8 17 11.8 10.1 18.2 13.2 15 25 15
y6 (1+) 8.4 6.7 7.1 8.3 16.3 13.4 11.8 17.6 15.2 15 25 15
sum 5.8 6.3 5.1 6.3 15.9 11 8.6 17.1 12.1 15 25 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-6102 Collapse assay details

Data source: Panorama

Official Gene Symbol
ILF2
Peptide Sequence
ILPTLEAVAALGNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
128
Peptide End
141
CPTAC ID
non-CPTAC-6102
Peptide Molecular Mass
1,408.8290
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 10.2 4.5 4.8 10.9 4.7 4.2 14.9 6.5 6.4 16 16 17
y12 (1+) 9.2 4.9 5.4 11.2 5.1 5.8 14.5 7.1 7.9 16 16 17
y6 (1+) 11.5 5.7 3.5 13.1 7.1 6.4 17.4 9.1 7.3 16 16 17
y9 (1+) 12.2 5.4 5.4 10.3 6.6 9.1 16 8.5 10.6 16 16 17
y12 (2+) 5.7 5.4 3 7.9 4.8 3.7 9.7 7.2 4.8 16 16 17
sum 5.5 4 2.5 7.2 3.5 3 9.1 5.3 3.9 16 16 17

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments