KPNA2, CPTAC-232 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
KPNA2	RCH1, SRP1

Sequence Length (AA)	Molecular Weight (Da)
529	57862

Protein Name
Importin subunit alpha-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSTNENANTP	AARLHRFKNK	GKDSTEMRRR	RIEVNVELRK	AKKDDQMLKR
60	70	80	90	100
RNVSSFPDDA	TSPLQENRNN	QGTVNWSVDD	IVKGINSSNV	ENQLQATQAA
110	120	130	140	150
RKLLSREKQP	PIDNIIRAGL	IPKFVSFLGR	TDCSPIQFES	AWALTNIASG
160	170	180	190	200
TSEQTKAVVD	GGAIPAFISL	LASPHAHISE	QAVWALGNIA	GDGSVFRDLV
210	220	230	240	250
IKYGAVDPLL	ALLAVPDMSS	LACGYLRNLT	WTLSNLCRNK	NPAPPIDAVE
260	270	280	290	300
QILPTLVRLL	HHDDPEVLAD	TCWAISYLTD	GPNERIGMVV	KTGVVPQLVK
310	320	330	340	350
LLGASELPIV	TPALRAIGNI	VTGTDEQTQV	VIDAGALAVF	PSLLTNPKTN
360	370	380	390	400
IQKEATWTMS	NITAGRQDQI	QQVVNHGLVP	FLVSVLSKAD	FKTQKEAVWA
410	420	430	440	450
VTNYTSGGTV	EQIVYLVHCG	IIEPLMNLLT	AKDTKIILVI	LDAISNIFQA
460	470	480	490	500
AEKLGETEKL	SIMIEECGGL	DKIEALQNHE	NESVYKASLS	LIEKYFSVEE
510	520	529
EEDQNVVPET	TSEGYTFQVQ	DGAPGTFNF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-233 Collapse assay details

Data source: Panorama

Official Gene Symbol: KPNA2
Peptide Sequence: GINSSNVENQLQATQAAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 84
Peptide End: 101

CPTAC ID: CPTAC-233
Peptide Molecular Mass: 1,899.9399
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	9.7	7.1	6	17.3	14.9	9	19.8	16.5	10.8	15	25	15
y10 (1+)	8.9	8.4	8.8	10.6	16.7	8.1	13.8	18.7	12	15	25	15
y9 (1+)	12.1	11.5	15.9	13.8	13.1	13.5	18.4	17.4	20.9	15	25	15
sum	8	5.4	5.4	12.3	14	6.9	14.7	15	8.8	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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