METTL7A, CPTAC-238 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
METTL7A	N/A

Sequence Length (AA)	Molecular Weight (Da)
244	28319

Protein Name
Methyltransferase-like protein 7A

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MELTIFILRL	AIYILTFPLY	LLNFLGLWSW	ICKKWFPYFL	VRFTVIYNEQ
60	70	80	90	100
MASKKRELFS	NLQEFAGPSG	KLSLLEVGCG	TGANFKFYPP	GCRVTCIDPN
110	120	130	140	150
PNFEKFLIKS	IAENRHLQFE	RFVVAAGENM	HQVADGSVDV	VVCTLVLCSV
160	170	180	190	200
KNQERILREV	CRVLRPGGAF	YFMEHVAAEC	STWNYFWQQV	LDPAWHLLFD
210	220	230	240	244
GCNLTRESWK	ALERASFSKL	KLQHIQAPLS	WELVRPHIYG	YAVK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-238 Collapse assay details

Data source: Panorama

Official Gene Symbol: METTL7A
Peptide Sequence: ELFSNLQEFAGPSGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 57
Peptide End: 71

CPTAC ID: CPTAC-238
Peptide Molecular Mass: 1,622.7940
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

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Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (1+)	13.5	10.9	10.5	16.6	12.2	12.2	21.4	16.4	16.1	15	25	15
y11 (1+)	12.1	13	9.8	13.4	12.8	12.3	18.1	18.2	15.7	15	25	15
y9 (1+)	12.6	11.4	15.5	17.6	12.1	16.1	21.6	16.6	22.3	15	25	15
sum	10.6	6.7	10	14.1	7.8	10.2	17.6	10.3	14.3	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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