OTUB1, CPTAC-244 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
OTUB1	OTB1, OTU1

Sequence Length (AA)	Molecular Weight (Da)
271	31284

Protein Name
Ubiquitin thioesterase OTUB1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAEEPQQQK	QEPLGSDSEG	VNCLAYDEAI	MAQQDRIQQE	IAVQNPLVSE
60	70	80	90	100
RLELSVLYKE	YAEDDNIYQQ	KIKDLHKKYS	YIRKTRPDGN	CFYRAFGFSH
110	120	130	140	150
LEALLDDSKE	LQRFKAVSAK	SKEDLVSQGF	TEFTIEDFHN	TFMDLIEQVE
160	170	180	190	200
KQTSVADLLA	SFNDQSTSDY	LVVYLRLLTS	GYLQRESKFF	EHFIEGGRTV
210	220	230	240	250
KEFCQQEVEP	MCKESDHIHI	IALAQALSVS	IQVEYMDRGE	GGTTNPHIFP
260	270	271
EGSEPKVYLL	YRPGHYDILY	K

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-244 Collapse assay details

Data source: Panorama

Official Gene Symbol: OTUB1
Peptide Sequence: IQQEIAVQNPLVSER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 37
Peptide End: 51

CPTAC ID: CPTAC-244
Peptide Molecular Mass: 1,722.9264
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	9	7.8	6.4	12.2	10.8	6.5	15.2	13.3	9.1	15	25	15
y9 (1+)	11.1	8.8	7.7	19	10.7	6.4	22	13.9	10	15	25	15
y8 (1+)	8.1	7.1	6.4	13.3	9.1	6.9	15.6	11.5	9.4	15	25	15
sum	6.1	7.3	6.1	11.5	10	6.1	13	12.4	8.6	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments