PPP2R1A, CPTAC-258 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PPP2R1A	N/A

Sequence Length (AA)	Molecular Weight (Da)
589	65309

Protein Name
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAADGDDSL	YPIAVLIDEL	RNEDVQLRLN	SIKKLSTIAL	ALGVERTRSE
60	70	80	90	100
LLPFLTDTIY	DEDEVLLALA	EQLGTFTTLV	GGPEYVHCLL	PPLESLATVE
110	120	130	140	150
ETVVRDKAVE	SLRAISHEHS	PSDLEAHFVP	LVKRLAGGDW	FTSRTSACGL
160	170	180	190	200
FSVCYPRVSS	AVKAELRQYF	RNLCSDDTPM	VRRAAASKLG	EFAKVLELDN
210	220	230	240	250
VKSEIIPMFS	NLASDEQDSV	RLLAVEACVN	IAQLLPQEDL	EALVMPTLRQ
260	270	280	290	300
AAEDKSWRVR	YMVADKFTEL	QKAVGPEITK	TDLVPAFQNL	MKDCEAEVRA
310	320	330	340	350
AASHKVKEFC	ENLSADCREN	VIMSQILPCI	KELVSDANQH	VKSALASVIM
360	370	380	390	400
GLSPILGKDN	TIEHLLPLFL	AQLKDECPEV	RLNIISNLDC	VNEVIGIRQL
410	420	430	440	450
SQSLLPAIVE	LAEDAKWRVR	LAIIEYMPLL	AGQLGVEFFD	EKLNSLCMAW
460	470	480	490	500
LVDHVYAIRE	AATSNLKKLV	EKFGKEWAHA	TIIPKVLAMS	GDPNYLHRMT
510	520	530	540	550
TLFCINVLSE	VCGQDITTKH	MLPTVLRMAG	DPVANVRFNV	AKSLQKIGPI
560	570	580	589
LDNSTLQSEV	KPILEKLTQD	QDVDVKYFAQ	EALTVLSLA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-257 Collapse assay details

Data source: Panorama

Official Gene Symbol: PPP2R1A
Peptide Modified Sequence: EFC[+57.0]ENLSADC[+57.0]R
Modification Type: Carbamidomethyl Cysteine, Carbamidomethyl Cysteine
Protein - Site of Modification: 310, 317
Peptide - Site of Modification: 3, 10
Peptide Start: 308
Peptide End: 318

CPTAC ID: CPTAC-257
Peptide Molecular Mass: 1,399.5497
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	10	12.2	26.7	29.9	21.9	42.1	31.5	25.1	49.9	15	25	15
y7 (1+)	10.5	12.2	26.6	30.6	21.2	44.3	32.4	24.5	51.7	15	25	15
y6 (1+)	14.5	10.2	27	30.7	20	45.9	34	22.5	53.3	15	25	15
sum	10.9	11	26.1	30.3	20.8	44.1	32.2	23.5	51.2	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments