Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
RALY HNRPCL2, P542
Sequence Length (AA) Molecular Weight (Da)
306 32463
Protein Name
RNA-binding protein Raly
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSLKLQASNV TNKNDPKSIN SRVFIGNLNT ALVKKSDVET IFSKYGRVAG
60 70 80 90 100
CSVHKGYAFV QYSNERHARA AVLGENGRVL AGQTLDINMA GEPKPDRPKG
110 120 130 140 150
LKRAASAIYS GYIFDYDYYR DDFYDRLFDY RGRLSPVPVP RAVPVKRPRV
160 170 180 190 200
TVPLVRRVKT NVPVKLFARS TAVTTSSAKI KLKSSELQAI KTELTQIKSN
210 220 230 240 250
IDALLSRLEQ IAAEQKANPD GKKKGDGGGA GGGGGGGGSG GGGSGGGGGG
260 270 280 290 300
GSSRPPAPQE NTTSEAGLPQ GEARTRDDGD EEGLLTHSEE ELEHSQDTDA
306
DDGALQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-264 Collapse assay details

Data source: Panorama

Official Gene Symbol
RALY
Peptide Sequence
GYAFVQYSNER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
56
Peptide End
66
CPTAC ID
CPTAC-264
Peptide Molecular Mass
1,332.6099
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 7.5 7.2 7.1 10.7 8.6 11.8 13.1 11.2 13.8 15 25 15
y7 (1+) 7.1 5.8 8.2 9.2 7.8 9.5 11.6 9.7 12.5 15 25 15
y6 (1+) 5.7 6.2 5.3 9.9 7.7 7.6 11.4 9.9 9.3 15 25 15
sum 5.4 5.5 5.8 8.4 7.2 8.2 10 9.1 10 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-265 Collapse assay details

Data source: Panorama

Official Gene Symbol
RALY
Peptide Sequence
SNIDALLSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
199
Peptide End
207
CPTAC ID
CPTAC-265
Peptide Molecular Mass
987.5349
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 17.8 19.5 13.7 29.1 28.9 30.1 34.1 34.9 33.1 15 25 15
y6 (1+) 5.4 5.4 5.1 7.4 6.7 5.6 9.2 8.6 7.6 15 25 15
y5 (1+) 8 7.7 5.6 8.3 8.8 6.9 11.5 11.7 8.9 15 25 15
sum 6.4 7.2 6.5 6.8 7.9 10.1 9.3 10.7 12 15 25 15

Additional Resources and Comments