RCN1, CPTAC-266 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RCN1	RCN

Sequence Length (AA)	Molecular Weight (Da)
331	38890

Protein Name
Reticulocalbin-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MARGGRGRRL	GLALGLLLAL	VLAPRVLRAK	PTVRKERVVR	PDSELGERPP
60	70	80	90	100
EDNQSFQYDH	EAFLGKEDSK	TFDQLTPDES	KERLGKIVDR	IDNDGDGFVT
110	120	130	140	150
TEELKTWIKR	VQKRYIFDNV	AKVWKDYDRD	KDDKISWEEY	KQATYGYYLG
160	170	180	190	200
NPAEFHDSSD	HHTFKKMLPR	DERRFKAADL	NGDLTATREE	FTAFLHPEEF
210	220	230	240	250
EHMKEIVVLE	TLEDIDKNGD	GFVDQDEYIA	DMFSHEENGP	EPDWVLSERE
260	270	280	290	300
QFNEFRDLNK	DGKLDKDEIR	HWILPQDYDH	AQAEARHLVY	ESDKNKDEKL
310	320	330	331
TKEEILENWN	MFVGSQATNY	GEDLTKNHDE	L

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-267 Collapse assay details

Data source: Panorama

Official Gene Symbol: RCN1
Peptide Sequence: EIVVLETLEDIDK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 205
Peptide End: 217

CPTAC ID: CPTAC-267
Peptide Molecular Mass: 1,514.8080
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	58.9	15.5	20.8	93.1	35.9	37.1	110.2	39.1	42.5	15	25	15
y9 (1+)	32.5	18.6	16.8	44.7	40.3	39.8	55.3	44.4	43.2	15	25	15
y8 (1+)	43.4	16.5	18.9	51.8	30.1	30.3	67.6	34.3	35.7	15	25	15
sum	32.5	12.6	14	50.9	32.8	32.3	60.4	35.1	35.2	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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