RUVBL1, CPTAC-268 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RUVBL1	INO80H, NMP238, TIP49, TIP49A

Sequence Length (AA)	Molecular Weight (Da)
456	50228

Protein Name
RuvB-like 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKIEEVKSTT	KTQRIASHSH	VKGLGLDESG	LAKQAASGLV	GQENAREACG
60	70	80	90	100
VIVELIKSKK	MAGRAVLLAG	PPGTGKTALA	LAIAQELGSK	VPFCPMVGSE
110	120	130	140	150
VYSTEIKKTE	VLMENFRRAI	GLRIKETKEV	YEGEVTELTP	CETENPMGGY
160	170	180	190	200
GKTISHVIIG	LKTAKGTKQL	KLDPSIFESL	QKERVEAGDV	IYIEANSGAV
210	220	230	240	250
KRQGRCDTYA	TEFDLEAEEY	VPLPKGDVHK	KKEIIQDVTL	HDLDVANARP
260	270	280	290	300
QGGQDILSMM	GQLMKPKKTE	ITDKLRGEIN	KVVNKYIDQG	IAELVPGVLF
310	320	330	340	350
VDEVHMLDIE	CFTYLHRALE	SSIAPIVIFA	SNRGNCVIRG	TEDITSPHGI
360	370	380	390	400
PLDLLDRVMI	IRTMLYTPQE	MKQIIKIRAQ	TEGINISEEA	LNHLGEIGTK
410	420	430	440	450
TTLRYSVQLL	TPANLLAKIN	GKDSIEKEHV	EEISELFYDA	KSSAKILADQ
456
QDKYMK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-268 Collapse assay details

Data source: Panorama

Official Gene Symbol: RUVBL1
Peptide Sequence: ALESSIAPIVIFASNR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 318
Peptide End: 333

CPTAC ID: CPTAC-268
Peptide Molecular Mass: 1,686.9305
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	14.2	7.7	6.6	14.5	13.2	8.3	20.3	15.3	10.6	15	25	15
y10 (1+)	7.8	7.2	4.9	15.6	11.5	6.5	17.4	13.6	8.1	15	25	15
y9 (1+)	5.9	5.8	4.8	15.4	10.6	6.9	16.5	12.1	8.4	15	25	15
sum	6.6	6	4.6	14.8	10.9	6.5	16.2	12.4	8	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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