RUVBL2, CPTAC-271 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RUVBL2	INO80J, TIP48, TIP49B

Sequence Length (AA)	Molecular Weight (Da)
463	51157

Protein Name
RuvB-like 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MATVTATTKV	PEIRDVTRIE	RIGAHSHIRG	LGLDDALEPR	QASQGMVGQL
60	70	80	90	100
AARRAAGVVL	EMIREGKIAG	RAVLIAGQPG	TGKTAIAMGM	AQALGPDTPF
110	120	130	140	150
TAIAGSEIFS	LEMSKTEALT	QAFRRSIGVR	IKEETEIIEG	EVVEIQIDRP
160	170	180	190	200
ATGTGSKVGK	LTLKTTEMET	IYDLGTKMIE	SLTKDKVQAG	DVITIDKATG
210	220	230	240	250
KISKLGRSFT	RARDYDAMGS	QTKFVQCPDG	ELQKRKEVVH	TVSLHEIDVI
260	270	280	290	300
NSRTQGFLAL	FSGDTGEIKS	EVREQINAKV	AEWREEGKAE	IIPGVLFIDE
310	320	330	340	350
VHMLDIESFS	FLNRALESDM	APVLIMATNR	GITRIRGTSY	QSPHGIPIDL
360	370	380	390	400
LDRLLIVSTT	PYSEKDTKQI	LRIRCEEEDV	EMSEDAYTVL	TRIGLETSLR
410	420	430	440	450
YAIQLITAAS	LVCRKRKGTE	VQVDDIKRVY	SLFLDESRST	QYMKEYQDAF
460	463
LFNELKGETM	DTS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-270 Collapse assay details

Data source: Panorama

Official Gene Symbol: RUVBL2
Peptide Sequence: GLGLDDALEPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 30
Peptide End: 40

CPTAC ID: CPTAC-270
Peptide Molecular Mass: 1,154.5932
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	6	6.5	5.9	15.4	8	6	16.5	10.3	8.4	15	25	15
y7 (1+)	5	5.5	5.1	7.3	6.9	5.7	8.8	8.8	7.6	15	25	15
y6 (1+)	8.2	7.3	3.9	11.5	8.8	5.1	14.1	11.4	6.4	15	25	15
sum	4.8	5.6	4.8	9.1	7.1	5.2	10.3	9	7.1	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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