SLC1A5, CPTAC-2714 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SLC1A5	ASCT2, M7V1, RDR, RDRC

Sequence Length (AA)	Molecular Weight (Da)
541	56598

Protein Name
Neutral amino acid transporter B(0)

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVADPPRDSK	GLAAAEPTAN	GGLALASIED	QGAAAGGYCG	SRDQVRRCLR
60	70	80	90	100
ANLLVLLTVV	AVVAGVALGL	GVSGAGGALA	LGPERLSAFV	FPGELLLRLL
110	120	130	140	150
RMIILPLVVC	SLIGGAASLD	PGALGRLGAW	ALLFFLVTTL	LASALGVGLA
160	170	180	190	200
LALQPGAASA	AINASVGAAG	SAENAPSKEV	LDSFLDLARN	IFPSNLVSAA
210	220	230	240	250
FRSYSTTYEE	RNITGTRVKV	PVGQEVEGMN	ILGLVVFAIV	FGVALRKLGP
260	270	280	290	300
EGELLIRFFN	SFNEATMVLV	SWIMWYAPVG	IMFLVAGKIV	EMEDVGLLFA
310	320	330	340	350
RLGKYILCCL	LGHAIHGLLV	LPLIYFLFTR	KNPYRFLWGI	VTPLATAFGT
360	370	380	390	400
SSSSATLPLM	MKCVEENNGV	AKHISRFILP	IGATVNMDGA	ALFQCVAAVF
410	420	430	440	450
IAQLSQQSLD	FVKIITILVT	ATASSVGAAG	IPAGGVLTLA	IILEAVNLPV
460	470	480	490	500
DHISLILAVD	WLVDRSCTVL	NVEGDALGAG	LLQNYVDRTE	SRSTEPELIQ
510	520	530	540	541
VKSELPLDPL	PVPTEEGNPL	LKHYRGPAGD	ATVASEKESV	M

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2714 Collapse assay details

Data source: Panorama

Official Gene Symbol: SLC1A5
Peptide Sequence: EVLDSFLDLAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 179
Peptide End: 189

CPTAC ID: CPTAC-2714
Peptide Molecular Mass: 1,276.6663
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher Q-Exactive
Internal Standard: 13 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 um x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	4.2	4.1	2.9	4.8	4.2	3.3	6.4	5.9	4.4	15	15	15
y4 (1+)	4.9	3.6	3.1	3.8	4	2.2	6.2	5.4	3.8	15	15	15
y5 (1+)	4.8	3.7	3	4.4	4	2.7	6.5	5.4	4	15	15	15
sum	4.3	3.6	2.8	4.1	3.8	2.6	5.9	5.2	3.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2713 Collapse assay details

Data source: Panorama

Official Gene Symbol: SLC1A5
Peptide Sequence: LSAFVFPGELLLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 86
Peptide End: 98

CPTAC ID: CPTAC-2713
Peptide Molecular Mass: 1,460.8391
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend