IDH2, CPTAC-2743 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IDH2	N/A

Sequence Length (AA)	Molecular Weight (Da)
452	50909

Protein Name
Isocitrate dehydrogenase [NADP], mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGYLRVVRS	LCRASGSRPA	WAPAALTAPT	SQEQPRRHYA	DKRIKVAKPV
60	70	80	90	100
VEMDGDEMTR	IIWQFIKEKL	ILPHVDIQLK	YFDLGLPNRD	QTDDQVTIDS
110	120	130	140	150
ALATQKYSVA	VKCATITPDE	ARVEEFKLKK	MWKSPNGTIR	NILGGTVFRE
160	170	180	190	200
PIICKNIPRL	VPGWTKPITI	GRHAHGDQYK	ATDFVADRAG	TFKMVFTPKD
210	220	230	240	250
GSGVKEWEVY	NFPAGGVGMG	MYNTDESISG	FAHSCFQYAI	QKKWPLYMST
260	270	280	290	300
KNTILKAYDG	RFKDIFQEIF	DKHYKTDFDK	NKIWYEHRLI	DDMVAQVLKS
310	320	330	340	350
SGGFVWACKN	YDGDVQSDIL	AQGFGSLGLM	TSVLVCPDGK	TIEAEAAHGT
360	370	380	390	400
VTRHYREHQK	GRPTSTNPIA	SIFAWTRGLE	HRGKLDGNQD	LIRFAQMLEK
410	420	430	440	450
VCVETVESGA	MTKDLAGCIH	GLSNVKLNEH	FLNTTDFLDT	IKSNLDRALG
452
RQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-222 Collapse assay details

Data source: Panorama

Official Gene Symbol: IDH2
Peptide Sequence: DQTDDQVTIDSALATQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 90
Peptide End: 106

CPTAC ID: CPTAC-222
Peptide Molecular Mass: 1,847.8749
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	18.3	9.3	13.2	30.9	20.4	19	35.9	22.4	23.1	15	25	15
y10 (1+)	11.1	7.5	8.6	27.9	18.3	22.9	30	19.8	24.5	15	25	15
y9 (1+)	14.7	13.6	15.2	33.5	19.3	25.1	36.6	23.6	29.3	15	25	15
sum	10.5	5.9	8.8	28	17.8	21.6	29.9	18.8	23.3	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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