PKLR, CPTAC-2748 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PKLR	PK1, PKL

Sequence Length (AA)	Molecular Weight (Da)
574	61830

Protein Name
Pyruvate kinase PKLR

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSIQENISSL	QLRSWVSKSQ	RDLAKSILIG	APGGPAGYLR	RASVAQLTQE
60	70	80	90	100
LGTAFFQQQQ	LPAAMADTFL	EHLCLLDIDS	EPVAARSTSI	IATIGPASRS
110	120	130	140	150
VERLKEMIKA	GMNIARLNFS	HGSHEYHAES	IANVREAVES	FAGSPLSYRP
160	170	180	190	200
VAIALDTKGP	EIRTGILQGG	PESEVELVKG	SQVLVTVDPA	FRTRGNANTV
210	220	230	240	250
WVDYPNIVRV	VPVGGRIYID	DGLISLVVQK	IGPEGLVTQV	ENGGVLGSRK
260	270	280	290	300
GVNLPGAQVD	LPGLSEQDVR	DLRFGVEHGV	DIVFASFVRK	ASDVAAVRAA
310	320	330	340	350
LGPEGHGIKI	ISKIENHEGV	KRFDEILEVS	DGIMVARGDL	GIEIPAEKVF
360	370	380	390	400
LAQKMMIGRC	NLAGKPVVCA	TQMLESMITK	PRPTRAETSD	VANAVLDGAD
410	420	430	440	450
CIMLSGETAK	GNFPVEAVKM	QHAIAREAEA	AVYHRQLFEE	LRRAAPLSRD
460	470	480	490	500
PTEVTAIGAV	EAAFKCCAAA	IIVLTTTGRS	AQLLSRYRPR	AAVIAVTRSA
510	520	530	540	550
QAARQVHLCR	GVFPLLYREP	PEAIWADDVD	RRVQFGIESG	KLRGFLRVGD
560	570	574
LVIVVTGWRP	GSGYTNIMRV	LSIS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2748 Collapse assay details

Data source: Panorama

Official Gene Symbol: PKLR
Peptide Sequence: GDLGIEIPAEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 295
Peptide End: 305

CPTAC ID: CPTAC-2748
Peptide Molecular Mass: 1,140.6027
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher Q-Exactive
Internal Standard: 13 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 um x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	8.4	4.6	5.5	8.7	5.3	4.2	12.1	7	6.9	15	15	15
y5 (1+)	24.8	9	5	35.3	9.6	5	43.1	13.2	7.1	15	15	15
y6 (1+)	15.1	6.6	6	22.2	5.4	4.1	26.8	8.5	7.3	15	15	15
sum	7.6	4.8	5.2	9.8	4.5	3.9	12.4	6.6	6.5	15	15	15

PKLR

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2748 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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PKLR

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2748 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments