SLC2A1, CPTAC-275 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SLC2A1	GLUT1

Sequence Length (AA)	Molecular Weight (Da)
492	54084

Protein Name
Solute carrier family 2, facilitated glucose transporter member 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEPSSKKLTG	RLMLAVGGAV	LGSLQFGYNT	GVINAPQKVI	EEFYNQTWVH
60	70	80	90	100
RYGESILPTT	LTTLWSLSVA	IFSVGGMIGS	FSVGLFVNRF	GRRNSMLMMN
110	120	130	140	150
LLAFVSAVLM	GFSKLGKSFE	MLILGRFIIG	VYCGLTTGFV	PMYVGEVSPT
160	170	180	190	200
ALRGALGTLH	QLGIVVGILI	AQVFGLDSIM	GNKDLWPLLL	SIIFIPALLQ
210	220	230	240	250
CIVLPFCPES	PRFLLINRNE	ENRAKSVLKK	LRGTADVTHD	LQEMKEESRQ
260	270	280	290	300
MMREKKVTIL	ELFRSPAYRQ	PILIAVVLQL	SQQLSGINAV	FYYSTSIFEK
310	320	330	340	350
AGVQQPVYAT	IGSGIVNTAF	TVVSLFVVER	AGRRTLHLIG	LAGMAGCAIL
360	370	380	390	400
MTIALALLEQ	LPWMSYLSIV	AIFGFVAFFE	VGPGPIPWFI	VAELFSQGPR
410	420	430	440	450
PAAIAVAGFS	NWTSNFIVGM	CFQYVEQLCG	PYVFIIFTVL	LVLFFIFTYF
460	470	480	490	492
KVPETKGRTF	DEIASGFRQG	GASQSDKTPE	ELFHPLGADS	QV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-275 Collapse assay details

Data source: Panorama

Official Gene Symbol: SLC2A1
Peptide Sequence: TFDEIASGFR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 459
Peptide End: 468

CPTAC ID: CPTAC-275
Peptide Molecular Mass: 1,141.5404
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	6	5.4	4.5	7	7.4	5.3	9.2	9.2	7	15	25	15
y7 (1+)	7.8	9.4	6.3	12.6	12.6	9.3	14.8	15.7	11.2	15	25	15
y6 (1+)	12	9.6	8.6	18.1	9.1	10.1	21.7	13.2	13.3	15	25	15
sum	5.1	5.1	4.8	8.5	6.8	5.7	9.9	8.5	7.5	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments