LDHA, CPTAC-2750 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
LDHA	N/A

Sequence Length (AA)	Molecular Weight (Da)
332	36689

Protein Name
L-lactate dehydrogenase A chain

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MATLKDQLIY	NLLKEEQTPQ	NKITVVGVGA	VGMACAISIL	MKDLADELAL
60	70	80	90	100
VDVIEDKLKG	EMMDLQHGSL	FLRTPKIVSG	KDYNVTANSK	LVIITAGARQ
110	120	130	140	150
QEGESRLNLV	QRNVNIFKFI	IPNVVKYSPN	CKLLIVSNPV	DILTYVAWKI
160	170	180	190	200
SGFPKNRVIG	SGCNLDSARF	RYLMGERLGV	HPLSCHGWVL	GEHGDSSVPV
210	220	230	240	250
WSGMNVAGVS	LKTLHPDLGT	DKDKEQWKEV	HKQVVESAYE	VIKLKGYTSW
260	270	280	290	300
AIGLSVADLA	ESIMKNLRRV	HPVSTMIKGL	YGIKDDVFLS	VPCILGQNGI
310	320	330	332
SDLVKVTLTS	EEEARLKKSA	DTLWGIQKEL	QF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-91 Collapse assay details

Data source: Panorama

Official Gene Symbol: LDHA
Peptide Sequence: DQLIYNLLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 6
Peptide End: 14

CPTAC ID: CPTAC-91
Peptide Molecular Mass: 1,118.6336
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N K

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	14.6	8.4	5.7	36.1	21.3	11	38.9	22.9	12.4	15	25	15
y6 (1+)	13.6	7.4	4.9	33.5	19.8	11	36.2	21.1	12	15	25	15
y5 (1+)	18.2	7.8	5.3	34.6	20.4	10.6	39.1	21.8	11.9	15	25	15
sum	14.6	7.7	5.1	33.8	20.3	10.6	36.8	21.7	11.8	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2749 Collapse assay details

Data source: Panorama

Official Gene Symbol: LDHA
Peptide Sequence: LVIITAGAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 91
Peptide End: 99

CPTAC ID: CPTAC-2749
Peptide Molecular Mass: 912.5757
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend