DLST, CPTAC-2756 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DLST	DLTS

Sequence Length (AA)	Molecular Weight (Da)
453	48755

Protein Name
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLSRSRCVSR	AFSRSLSAFQ	KGNCPLGRRS	LPGVSLCQGP	GYPNSRKVVI
60	70	80	90	100
NNSVFSVRFF	RTTAVCKDDL	VTVKTPAFAE	SVTEGDVRWE	KAVGDTVAED
110	120	130	140	150
EVVCEIETDK	TSVQVPSPAN	GVIEALLVPD	GGKVEGGTPL	FTLRKTGAAP
160	170	180	190	200
AKAKPAEAPA	AAAPKAEPTA	AAVPPPAAPI	PTQMPPVPSP	SQPPSGKPVS
210	220	230	240	250
AVKPTVAPPL	AEPGAGKGLR	SEHREKMNRM	RQRIAQRLKE	AQNTCAMLTT
260	270	280	290	300
FNEIDMSNIQ	EMRARHKEAF	LKKHNLKLGF	MSAFVKASAF	ALQEQPVVNA
310	320	330	340	350
VIDDTTKEVV	YRDYIDISVA	VATPRGLVVP	VIRNVEAMNF	ADIERTITEL
360	370	380	390	400
GEKARKNELA	IEDMDGGTFT	ISNGGVFGSL	FGTPIINPPQ	SAILGMHGIF
410	420	430	440	450
DRPVAIGGKV	EVRPMMYVAL	TYDHRLIDGR	EAVTFLRKIK	AAVEDPRVLL
453
LDL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2756 Collapse assay details

Data source: Panorama

Official Gene Symbol: DLST
Peptide Sequence: VEGGTPLFTLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 134
Peptide End: 144

CPTAC ID: CPTAC-2756
Peptide Molecular Mass: 1,188.6503
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher Q-Exactive
Internal Standard: 13 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 um x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	51.8	16.8	21	50.9	14.8	27.4	72.6	22.4	34.5	15	15	15
y4 (1+)	31.8	11.5	8.2	31.2	11.6	7.4	44.5	16.3	11	15	15	15
y5 (1+)	69.4	17.9	15	76.3	19.9	11	103.1	26.8	18.6	15	15	15
sum	34.6	12.1	8.2	39.9	11.9	8.5	52.8	17	11.8	15	15	15

DLST

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2756 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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DLST

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2756 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments