PSPH, CPTAC-2765 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PSPH	N/A

Sequence Length (AA)	Molecular Weight (Da)
225	25008

Protein Name
Phosphoserine phosphatase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVSHSELRKL	FYSADAVCFD	VDSTVIREEG	IDELAKICGV	EDAVSEMTRR
60	70	80	90	100
AMGGAVPFKA	ALTERLALIQ	PSREQVQRLI	AEQPPHLTPG	IRELVSRLQE
110	120	130	140	150
RNVQVFLISG	GFRSIVEHVA	SKLNIPATNV	FANRLKFYFN	GEYAGFDETQ
160	170	180	190	200
PTAESGGKGK	VIKLLKEKFH	FKKIIMIGDG	ATDMEACPPA	DAFIGFGGNV
210	220	225
IRQQVKDNAK	WYITDFVELL	GELEE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2765 Collapse assay details

Data source: Panorama

Official Gene Symbol: PSPH
Peptide Sequence: LALIQPSR
Modification Type
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 65
Peptide End: 73

CPTAC ID: CPTAC-2765
Peptide Molecular Mass: 896.5444
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher Q-Exactive
Internal Standard: 13 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 um x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	5.8	5.7	3.8	7	7	3.8	9.1	9	5.4	15	15	15
y4 (1+)	3.5	5.2	6	6.8	6.7	5.6	7.6	8.5	8.2	15	15	15
y3 (1+)	5.2	5.6	6	5.6	5.9	5.5	7.6	8.1	8.1	15	15	15
sum	3	4.7	5.3	5.3	5.7	4.9	6.1	7.4	7.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2766 Collapse assay details

Data source: Panorama

Official Gene Symbol: PSPH
Peptide Sequence: LNIPATNVFANR
Modification Type
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 122
Peptide End: 134

CPTAC ID: CPTAC-2766
Peptide Molecular Mass: 1,328.7201
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend