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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Rpia Rpi
Sequence Length (AA) Molecular Weight (Da)
303 32451
Protein Name
Ribose-5-phosphate isomerase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQRPGPFSTL YGRVLAPLPG RAGGAASGGG GNNWGLSGSH VQLPGRAHSE
60 70 80 90 100
TRGDKGGSSA GGPAPSTMSK AEEAKKLASH TAVENHVKNN QVLGIGSGST
110 120 130 140 150
IVHAVQRIAE RVKQENLDLI CIPTSFQARQ LILQYGLTLS DLDQHPEIDL
160 170 180 190 200
AIDGADEVDA ELNLIKGGGG CLTQEKIVAG YASRFIVIAD FRKDSKNLGD
210 220 230 240 250
RWHKGIPIEV IPMAYVPVSR AVAQKFGGEV ELRMAVNKAG PVVTDNGNFI
260 270 280 290 300
LDWKFDRVHK WSEVNTAIKM TPGVVDTGLF INMAERVYFG MQDGSVNVRE
303
KPF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2767 Collapse assay details

Data source: Panorama

Official Gene Symbol
Rpia
Peptide Sequence
FIVIADFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
185
Peptide End
192
CPTAC ID
CPTAC-2767
Peptide Molecular Mass
979.5491
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher Q-Exactive
Internal Standard
13 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 um x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 27.5 7.2 10 23.2 7.2 11.2 36 10.2 15 15 15 15
y5 (1+) 8.3 3.3 7 12.1 2.7 5.8 14.7 4.3 9.1 15 15 15
y6 (1+) 8.6 2.8 6.1 9.6 2.7 6.1 12.9 3.9 8.6 15 15 15
sum 8 2.6 6.4 9.8 2.5 5.9 12.7 3.6 8.7 15 15 15


Additional Resources and Comments