PSAT1, CPTAC-2773 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PSAT1	PSA

Sequence Length (AA)	Molecular Weight (Da)
370	40423

Protein Name
Phosphoserine aminotransferase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDAPRQVVNF	GPGPAKLPHS	VLLEIQKELL	DYKGVGISVL	EMSHRSSDFA
60	70	80	90	100
KIINNTENLV	RELLAVPDNY	KVIFLQGGGC	GQFSAVPLNL	IGLKAGRCAD
110	120	130	140	150
YVVTGAWSAK	AAEEAKKFGT	INIVHPKLGS	YTKIPDPSTW	NLNPDASYVY
160	170	180	190	200
YCANETVHGV	EFDFIPDVKG	AVLVCDMSSN	FLSKPVDVSK	FGVIFAGAQK
210	220	230	240	250
NVGSAGVTVV	IVRDDLLGFA	LRECPSVLEY	KVQAGNSSLY	NTPPCFSIYV
260	270	280	290	300
MGLVLEWIKN	NGGAAAMEKL	SSIKSQTIYE	IIDNSQGFYV	CPVEPQNRSK
310	320	330	340	350
MNIPFRIGNA	KGDDALEKRF	LDKALELNML	SLKGHRSVGG	IRASLYNAVT
360	370
IEDVQKLAAF	MKKFLEMHQL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2775 Collapse assay details

Data source: Panorama

Official Gene Symbol: PSAT1
Peptide Sequence: ASLYNAVTIEDVQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 343
Peptide End: 356

CPTAC ID: CPTAC-2775
Peptide Molecular Mass: 1,549.7988
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher Q-Exactive
Internal Standard: 13 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 um x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	28	6.2	7.3	22.6	6.8	7.2	36	9.2	10.3	15	15	15
y4 (1+)	24.2	6.1	4.3	21.7	8.7	6.9	32.5	10.6	8.1	15	15	15
y3 (1+)	36.6	6.8	8.3	30.9	8.9	8.4	47.9	11.2	11.8	15	15	15
sum	28.2	4.1	5.6	22.6	6.3	6.2	36.1	7.5	8.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2774 Collapse assay details

Data source: Panorama

Official Gene Symbol: PSAT1
Peptide Sequence: FGVIFAGAQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 191
Peptide End: 200

CPTAC ID: CPTAC-2774
Peptide Molecular Mass: 1,036.5706
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend