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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SUCLG2 N/A
Sequence Length (AA) Molecular Weight (Da)
432 46511
Protein Name
Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASPVAAQAG KLLRALALRP RFLAAGSQAV QLTSRRWLNL QEYQSKKLMS
60 70 80 90 100
DNGVRVQRFF VADTANEALE AAKRLNAKEI VLKAQILAGG RGKGVFNSGL
110 120 130 140 150
KGGVHLTKDP NVVGQLAKQM IGYNLATKQT PKEGVKVNKV MVAEALDISR
160 170 180 190 200
ETYLAILMDR SCNGPVLVGS PQGGVDIEEV AASNPELIFK EQIDIFEGIK
210 220 230 240 250
DSQAQRMAEN LGFVGPLKSQ AADQITKLYN LFLKIDATQV EVNPFGETPE
260 270 280 290 300
GQVVCFDAKI NFDDNAEFRQ KDIFAMDDKS ENEPIENEAA KYDLKYIGLD
310 320 330 340 350
GNIACFVNGA GLAMATCDII FLNGGKPANF LDLGGGVKEA QVYQAFKLLT
360 370 380 390 400
ADPKVEAILV NIFGGIVNCA IIANGITKAC RELELKVPLV VRLEGTNVQE
410 420 430 432
AQKILNNSGL PITSAIDLED AAKKAVASVA KK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2776 Collapse assay details

Data source: Panorama

Official Gene Symbol
SUCLG2
Peptide Sequence
INFDDNAEFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
260
Peptide End
269
CPTAC ID
CPTAC-2776
Peptide Molecular Mass
1,239.5520
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher Q-Exactive
Internal Standard
13 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 um x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 7.9 5.7 1.9 9.7 6.7 3.7 12.5 8.8 4.2 15 15 15
y4 (1+) 13 7.8 7.7 17.6 6.9 10.5 21.9 10.4 13 15 15 15
y3 (1+) 12.1 4.6 5.6 21.1 6.3 7.6 24.3 7.8 9.4 15 15 15
sum 6 4.1 3.1 9.9 5.6 4.5 11.6 6.9 5.5 15 15 15


Additional Resources and Comments