CCNE2, CPTAC-2796 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CCNE2	N/A

Sequence Length (AA)	Molecular Weight (Da)
404	46757

Protein Name
G1/S-specific cyclin-E2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSRRSSRLQA	KQQPQPSQTE	SPQEAQIIQA	KKRKTTQDVK	KRREEVTKKH
60	70	80	90	100
QYEIRNCWPP	VLSGGISPCI	IIETPHKEIG	TSDFSRFTNY	RFKNLFINPS
110	120	130	140	150
PLPDLSWGCS	KEVWLNMLKK	ESRYVHDKHF	EVLHSDLEPQ	MRSILLDWLL
160	170	180	190	200
EVCEVYTLHR	ETFYLAQDFF	DRFMLTQKDI	NKNMLQLIGI	TSLFIASKLE
210	220	230	240	250
EIYAPKLQEF	AYVTDGACSE	EDILRMELII	LKALKWELCP	VTIISWLNLF
260	270	280	290	300
LQVDALKDAP	KVLLPQYSQE	TFIQIAQLLD	LCILAIDSLE	FQYRILTAAA
310	320	330	340	350
LCHFTSIEVV	KKASGLEWDS	ISECVDWMVP	FVNVVKSTSP	VKLKTFKKIP
360	370	380	390	400
MEDRHNIQTH	TNYLAMLEEV	NYINTFRKGG	QLSPVCNGGI	MTPPKSTEKP
404
PGKH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2796 Collapse assay details

Data source: Panorama

Official Gene Symbol: CCNE2
Peptide Sequence: EIGTSDFSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 78
Peptide End: 86

CPTAC ID: CPTAC-2796
Peptide Molecular Mass: 1,010.4669
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	14.4	11.1	14.8	16.1	12.1	14.3	21.6	16.4	20.6	15	15	15
y4 (1+)	11.6	11.2	12.3	14.5	12.4	12.1	18.6	16.7	17.3	15	15	15
y3 (1+)	12.8	10.4	13.3	17	12.2	12.2	21.3	16	18	15	15	15
sum	13.3	10.8	13.9	16	12.1	13.2	20.8	16.2	19.2	15	15	15

CCNE2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2796 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CCNE2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2796 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments