CCNC, CPTAC-2806 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CCNC	N/A

Sequence Length (AA)	Molecular Weight (Da)
283	33243

Protein Name
Cyclin-C

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGNFWQSSH	YLQWILDKQD	LLKERQKDLK	FLSEEEYWKL	QIFFTNVIQA
60	70	80	90	100
LGEHLKLRQQ	VIATATVYFK	RFYARYSLKS	IDPVLMAPTC	VFLASKVEEF
110	120	130	140	150
GVVSNTRLIA	AATSVLKTRF	SYAFPKEFPY	RMNHILECEF	YLLELMDCCL
160	170	180	190	200
IVYHPYRPLL	QYVQDMGQED	MLLPLAWRIV	NDTYRTDLCL	LYPPFMIALA
210	220	230	240	250
CLHVACVVQQ	KDARQWFAEL	SVDMEKILEI	IRVILKLYEQ	WKNFDERKEM
260	270	280	283
ATILSKMPKP	KPPPNSEGEQ	GPNGSQNSSY	SQS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2806 Collapse assay details

Data source: Panorama

Official Gene Symbol: CCNC
Peptide Sequence: LIAAATSVLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 108
Peptide End: 117

CPTAC ID: CPTAC-2806
Peptide Molecular Mass: 985.6172
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	7.6	9.1	7.3	6.2	11.1	8.7	9.8	14.4	11.4	15	15	15
y7 (1+)	5.8	8	6.7	7.3	10.8	6.2	9.3	13.4	9.1	15	15	15
y6 (1+)	8.4	9.3	9.2	8.4	10.5	10	11.9	14	13.6	15	15	15
sum	7	8.6	7.2	6	10.7	8.2	9.2	13.7	10.9	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2807 Collapse assay details

Data source: Panorama

Official Gene Symbol: CCNC
Peptide Sequence: VEEFGVVSNTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 97
Peptide End: 107

CPTAC ID: CPTAC-2807
Peptide Molecular Mass: 1,235.6146
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend