CCND3, CPTAC-2809 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CCND3	N/A

Sequence Length (AA)	Molecular Weight (Da)
292	32520

Protein Name
G1/S-specific cyclin-D3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MELLCCEGTR	HAPRAGPDPR	LLGDQRVLQS	LLRLEERYVP	RASYFQCVQR
60	70	80	90	100
EIKPHMRKML	AYWMLEVCEE	QRCEEEVFPL	AMNYLDRYLS	CVPTRKAQLQ
110	120	130	140	150
LLGAVCMLLA	SKLRETTPLT	IEKLCIYTDH	AVSPRQLRDW	EVLVLGKLKW
160	170	180	190	200
DLAAVIAHDF	LAFILHRLSL	PRDRQALVKK	HAQTFLALCA	TDYTFAMYPP
210	220	230	240	250
SMIATGSIGA	AVQGLGACSM	SGDELTELLA	GITGTEVDCL	RACQEQIEAA
260	270	280	290	292
LRESLREASQ	TSSSPAPKAP	RGSSSQGPSQ	TSTPTDVTAI	HL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2809 Collapse assay details

Data source: Panorama

Official Gene Symbol: CCND3
Peptide Modified Sequence: ASYFQC[+57.0]VQR
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 47
Peptide - Site of Modification: 6
Peptide Start: 42
Peptide End: 50

CPTAC ID: CPTAC-2809
Peptide Molecular Mass: 1,157.5288
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	14.2	11.2	11.9	14.3	10.4	16	20.2	15.3	19.9	15	15	15
y5 (1+)	13.2	9.8	10.7	14.8	9.7	12	19.8	13.8	16.1	15	15	15
y4 (1+)	10.8	12.1	12	11.9	11.4	13	16.1	16.6	17.7	15	15	15
sum	12.4	10.8	11.2	13.6	10.1	12.5	18.4	14.8	16.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2808 Collapse assay details

Data source: Panorama

Official Gene Symbol: CCND3
Peptide Modified Sequence: LC[+57.0]IYTDHAVSPR
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 125
Peptide - Site of Modification: 2
Peptide Start: 124
Peptide End: 135

CPTAC ID: CPTAC-2808
Peptide Molecular Mass: 1,430.6976
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend