Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TOM1L1 SRCASM
Sequence Length (AA) Molecular Weight (Da)
476 52989
Protein Name
TOM1-like protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAFGKSHRDP YATSVGHLIE KATFAGVQTE DWGQFMHICD IINTTQDGPK
60 70 80 90 100
DAVKALKKRI SKNYNHKEIQ LTLSLIDMCV QNCGPSFQSL IVKKEFVKEN
110 120 130 140 150
LVKLLNPRYN LPLDIQNRIL NFIKTWSQGF PGGVDVSEVK EVYLDLVKKG
160 170 180 190 200
VQFPPSEAEA ETARQETAQI SSNPPTSVPT APALSSVIAP KNSTVTLVPE
210 220 230 240 250
QIGKLHSELD MVKMNVRVMS AILMENTPGS ENHEDIELLQ KLYKTGREMQ
260 270 280 290 300
ERIMDLLVVV ENEDVTVELI QVNEDLNNAI LGYERFTRNQ QRILEQNKNQ
310 320 330 340 350
KEATNTTSEP SAPSQDLLDL SPSPRMPRAT LGELNTMNNQ LSGLNFSLPS
360 370 380 390 400
SDVTNNLKPS LHPQMNLLAL ENTEIPPFAQ RTSQNLTSSH AYDNFLEHSN
410 420 430 440 450
SVFLQPVSLQ TIAAAPSNQS LPPLPSNHPA MTKSDLQPPN YYEVMEFDPL
460 470 476
APAVTTEAIY EEIDAHQHKG AQNDGD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-285 Collapse assay details

Data source: Panorama

Official Gene Symbol
TOM1L1
Peptide Sequence
NSTVTLVPEQIGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
192
Peptide End
204
CPTAC ID
CPTAC-285
Peptide Molecular Mass
1,384.7562
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 10.2 10.2 9.2 12.3 10.4 10.5 16 14.6 14 15 25 15
y8 (1+) 31.1 27.2 24.1 38 24.6 23.3 49.1 36.7 33.5 15 25 15
y7 (1+) 11.5 9 7.3 8.5 12.6 9.1 14.3 15.5 11.7 15 25 15
sum 6.5 7.2 6 6.8 7.1 6.1 9.4 10.1 8.6 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-284 Collapse assay details

Data source: Panorama

Official Gene Symbol
TOM1L1
Peptide Sequence
TWSQGFPGGVDVSEVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
125
Peptide End
140
CPTAC ID
CPTAC-284
Peptide Molecular Mass
1,691.8155
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 27 19.6 18.5 40.7 24.1 17.2 48.8 31.1 25.3 15 25 15
y11 (1+) 30.7 51.1 43.9 46.9 52.8 40.6 56.1 73.5 59.8 15 25 15
y10 (1+) 9.2 11.5 8.4 23.4 16.3 19.6 25.1 19.9 21.3 15 25 15
sum 8.7 10.8 11 25.7 14.8 16.9 27.1 18.3 20.2 15 25 15

Additional Resources and Comments