TRAP1, CPTAC-288 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TRAP1	HSP75

Sequence Length (AA)	Molecular Weight (Da)
704	80110

Protein Name
Heat shock protein 75 kDa, mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MARELRALLL	WGRRLRPLLR	APALAAVPGG	KPILCPRRTT	AQLGPRRNPA
60	70	80	90	100
WSLQAGRLFS	TQTAEDKEEP	LHSIISSTES	VQGSTSKHEF	QAETKKLLDI
110	120	130	140	150
VARSLYSEKE	VFIRELISNA	SDALEKLRHK	LVSDGQALPE	MEIHLQTNAE
160	170	180	190	200
KGTITIQDTG	IGMTQEELVS	NLGTIARSGS	KAFLDALQNQ	AEASSKIIGQ
210	220	230	240	250
FGVGFYSAFM	VADRVEVYSR	SAAPGSLGYQ	WLSDGSGVFE	IAEASGVRTG
260	270	280	290	300
TKIIIHLKSD	CKEFSSEARV	RDVVTKYSNF	VSFPLYLNGR	RMNTLQAIWM
310	320	330	340	350
MDPKDVREWQ	HEEFYRYVAQ	AHDKPRYTLH	YKTDAPLNIR	SIFYVPDMKP
360	370	380	390	400
SMFDVSRELG	SSVALYSRKV	LIQTKATDIL	PKWLRFIRGV	VDSEDIPLNL
410	420	430	440	450
SRELLQESAL	IRKLRDVLQQ	RLIKFFIDQS	KKDAEKYAKF	FEDYGLFMRE
460	470	480	490	500
GIVTATEQEV	KEDIAKLLRY	ESSALPSGQL	TSLSEYASRM	RAGTRNIYYL
510	520	530	540	550
CAPNRHLAEH	SPYYEAMKKK	DTEVLFCFEQ	FDELTLLHLR	EFDKKKLISV
560	570	580	590	600
ETDIVVDHYK	EEKFEDRSPA	AECLSEKETE	ELMAWMRNVL	GSRVTNVKVT
610	620	630	640	650
LRLDTHPAMV	TVLEMGAARH	FLRMQQLAKT	QEERAQLLQP	TLEINPRHAL
660	670	680	690	700
IKKLNQLRAS	EPGLAQLLVD	QIYENAMIAA	GLVDDPRAMV	GRLNELLVKA
704
LERH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-289 Collapse assay details

Data source: Panorama

Official Gene Symbol: TRAP1
Peptide Sequence: AFLDALQNQAEASSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 182
Peptide End: 196

CPTAC ID: CPTAC-289
Peptide Molecular Mass: 1,591.7842
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	7	7.8	5.6	12	9.9	5.6	13.9	12.6	7.9	15	25	15
y9 (1+)	5.8	7	6.4	10.7	9.5	9.4	12.2	11.8	11.4	15	25	15
y8 (1+)	8.8	6.1	6.5	12.8	8.6	7.6	15.5	10.5	10	15	25	15
sum	4.4	5.8	5.4	9.8	8.2	7	10.7	10	8.8	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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