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Overview Data source: UniProt

Official Gene Symbol Other Aliases
TRAP1 HSP75
Sequence Length (AA) Molecular Weight (Da)
704 80110
Protein Name
Heat shock protein 75 kDa, mitochondrial
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MARELRALLL WGRRLRPLLR APALAAVPGG KPILCPRRTT AQLGPRRNPA
60 70 80 90 100
WSLQAGRLFS TQTAEDKEEP LHSIISSTES VQGSTSKHEF QAETKKLLDI
110 120 130 140 150
VARSLYSEKE VFIRELISNA SDALEKLRHK LVSDGQALPE MEIHLQTNAE
160 170 180 190 200
KGTITIQDTG IGMTQEELVS NLGTIARSGS KAFLDALQNQ AEASSKIIGQ
210 220 230 240 250
FGVGFYSAFM VADRVEVYSR SAAPGSLGYQ WLSDGSGVFE IAEASGVRTG
260 270 280 290 300
TKIIIHLKSD CKEFSSEARV RDVVTKYSNF VSFPLYLNGR RMNTLQAIWM
310 320 330 340 350
MDPKDVREWQ HEEFYRYVAQ AHDKPRYTLH YKTDAPLNIR SIFYVPDMKP
360 370 380 390 400
SMFDVSRELG SSVALYSRKV LIQTKATDIL PKWLRFIRGV VDSEDIPLNL
410 420 430 440 450
SRELLQESAL IRKLRDVLQQ RLIKFFIDQS KKDAEKYAKF FEDYGLFMRE
460 470 480 490 500
GIVTATEQEV KEDIAKLLRY ESSALPSGQL TSLSEYASRM RAGTRNIYYL
510 520 530 540 550
CAPNRHLAEH SPYYEAMKKK DTEVLFCFEQ FDELTLLHLR EFDKKKLISV
560 570 580 590 600
ETDIVVDHYK EEKFEDRSPA AECLSEKETE ELMAWMRNVL GSRVTNVKVT
610 620 630 640 650
LRLDTHPAMV TVLEMGAARH FLRMQQLAKT QEERAQLLQP TLEINPRHAL
660 670 680 690 700
IKKLNQLRAS EPGLAQLLVD QIYENAMIAA GLVDDPRAMV GRLNELLVKA
704
LERH

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-289 Collapse assay details

Data source: Panorama

Official Gene Symbol
TRAP1
Peptide Sequence
AFLDALQNQAEASSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
182
Peptide End
196
CPTAC ID
CPTAC-289
Peptide Molecular Mass
1,591.7842
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 7 7.8 5.6 12 9.9 5.6 13.9 12.6 7.9 15 25 15
y9 (1+) 5.8 7 6.4 10.7 9.5 9.4 12.2 11.8 11.4 15 25 15
y8 (1+) 8.8 6.1 6.5 12.8 8.6 7.6 15.5 10.5 10 15 25 15
sum 4.4 5.8 5.4 9.8 8.2 7 10.7 10 8.8 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-288 Collapse assay details

Data source: Panorama

Official Gene Symbol
TRAP1
Peptide Sequence
LFSTQTAEDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
58
Peptide End
67
CPTAC ID
CPTAC-288
Peptide Molecular Mass
1,138.5506
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 3.7 2.5 2.1 3.4 3.7 3 5 4.5 3.7 15 15 15
y7 (1+) 14.2 8.5 4.5 57.7 8.5 5.2 59.4 12 6.9 15 15 15
y6 (1+) 10.1 6.5 7.7 11.4 7.5 6.7 15.2 9.9 10.2 15 15 15
sum 5.3 2.3 2.3 14.5 3.2 2.7 15.4 3.9 3.5 15 15 15

Additional Resources and Comments