UGGT1, CPTAC-290 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
UGGT1	GT, UGCGL1, UGGT, UGT1, UGTR

Sequence Length (AA)	Molecular Weight (Da)
1555	177190

Protein Name
UDP-glucose:glycoprotein glucosyltransferase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGCKGDASGA	CAAGALPVTG	VCYKMGVLVV	LTVLWLFSSV	KADSKAITTS
60	70	80	90	100
LTTKWFSTPL	LLEASEFLAE	DSQEKFWNFV	EASQNIGSSD	HDGTDYSYYH
110	120	130	140	150
AILEAAFQFL	SPLQQNLFKF	CLSLRSYSAT	IQAFQQIAAD	EPPPEGCNSF
160	170	180	190	200
FSVHGKKTCE	SDTLEALLLT	ASERPKPLLF	KGDHRYPSSN	PESPVVIFYS
210	220	230	240	250
EIGSEEFSNF	HRQLISKSNA	GKINYVFRHY	IFNPRKEPVY	LSGYGVELAI
260	270	280	290	300
KSTEYKAKDD	TQVKGTEVNT	TVIGENDPID	EVQGFLFGKL	RDLHPDLEGQ
310	320	330	340	350
LKELRKHLVE	STNEMAPLKV	WQLQDLSFQT	AARILASPVE	LALVVMKDLS
360	370	380	390	400
QNFPTKARAI	TKTAVSSELR	TEVEENQKYF	KGTLGLQPGD	SALFINGLHM
410	420	430	440	450
DLDTQDIFSL	FDVLRNEARV	MEGLHRLGIE	GLSLHNVLKL	NIQPSEADYA
460	470	480	490	500
VDIRSPAISW	VNNLEVDSRY	NSWPSSLQEL	LRPTFPGVIR	QIRKNLHNMV
510	520	530	540	550
FIVDPAHETT	AELMNTAEMF	LSNHIPLRIG	FIFVVNDSED	VDGMQDAGVA
560	570	580	590	600
VLRAYNYVAQ	EVDDYHAFQT	LTHIYNKVRT	GEKVKVEHVV	SVLEKKYPYV
610	620	630	640	650
EVNSILGIDS	AYDRNRKEAR	GYYEQTGVGP	LPVVLFNGMP	FEREQLDPDE
660	670	680	690	700
LETITMHKIL	ETTTFFQRAV	YLGELPHDQD	VVEYIMNQPN	VVPRINSRIL
710	720	730	740	750
TAERDYLDLT	ASNNFFVDDY	ARFTILDSQG	KTAAVANSMN	YLTKKGMSSK
760	770	780	790	800
EIYDDSFIRP	VTFWIVGDFD	SPSGRQLLYD	AIKHQKSSNN	VRISMINNPA
810	820	830	840	850
KEISYENTQI	SRAIWAALQT	QTSNAAKNFI	TKMAKEGAAE	ALAAGADIAE
860	870	880	890	900
FSVGGMDFSL	FKEVFESSKM	DFILSHAVYC	RDVLKLKKGQ	RAVISNGRII
910	920	930	940	950
GPLEDSELFN	QDDFHLLENI	ILKTSGQKIK	SHIQQLRVEE	DVASDLVMKV
960	970	980	990	1000
DALLSAQPKG	DPRIEYQFFE	DRHSAIKLRP	KEGETYFDVV	AVVDPVTREA
1010	1020	1030	1040	1050
QRLAPLLLVL	AQLINMNLRV	FMNCQSKLSD	MPLKSFYRYV	LEPEISFTSD
1060	1070	1080	1090	1100
NSFAKGPIAK	FLDMPQSPLF	TLNLNTPESW	MVESVRTPYD	LDNIYLEEVD
1110	1120	1130	1140	1150
SVVAAEYELE	YLLLEGHCYD	ITTGQPPRGL	QFTLGTSANP	VIVDTIVMAN
1160	1170	1180	1190	1200
LGYFQLKANP	GAWILRLRKG	RSEDIYRIYS	HDGTDSPPDA	DEVVIVLNNF
1210	1220	1230	1240	1250
KSKIIKVKVQ	KKADMVNEDL	LSDGTSENES	GFWDSFKWGF	TGQKTEEVKQ
1260	1270	1280	1290	1300
DKDDIINIFS	VASGHLYERF	LRIMMLSVLK	NTKTPVKFWF	LKNYLSPTFK
1310	1320	1330	1340	1350
EFIPYMANEY	NFQYELVQYK	WPRWLHQQTE	KQRIIWGYKI	LFLDVLFPLV
1360	1370	1380	1390	1400
VDKFLFVDAD	QIVRTDLKEL	RDFNLDGAPY	GYTPFCDSRR	EMDGYRFWKS
1410	1420	1430	1440	1450
GYWASHLAGR	KYHISALYVV	DLKKFRKIAA	GDRLRGQYQG	LSQDPNSLSN
1460	1470	1480	1490	1500
LDQDLPNNMI	HQVPIKSLPQ	EWLWCETWCD	DASKKRAKTI	DLCNNPMTKE
1510	1520	1530	1540	1550
PKLEAAVRIV	PEWQDYDQEI	KQLQIRFQKE	KETGALYKEK	TKEPSREGPQ
1555
KREEL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-290 Collapse assay details

Data source: Panorama

Official Gene Symbol: UGGT1
Peptide Sequence: VWQLQDLSFQTAAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 320
Peptide End: 333

CPTAC ID: CPTAC-290
Peptide Molecular Mass: 1,661.8526
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	9.7	7	7.4	14.1	15.2	10	17.1	16.7	12.4	15	25	15
y9 (1+)	15.6	11.5	8.6	25	20	11.6	29.5	23.1	14.4	15	25	15
y8 (1+)	18.5	17.6	9.8	18.7	18.6	15.8	26.3	25.6	18.6	15	25	15
sum	10	7.8	5.7	16	16.5	9.6	18.9	18.3	11.2	15	25	15

UGGT1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-290 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

UGGT1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-290 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments