UGP2, CPTAC-292 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
UGP2	UGP1

Sequence Length (AA)	Molecular Weight (Da)
508	56940

Protein Name
UTP--glucose-1-phosphate uridylyltransferase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSRFVQDLSK	AMSQDGASQF	QEVIRQELEL	SVKKELEKIL	TTASSHEFEH
60	70	80	90	100
TKKDLDGFRK	LFHRFLQEKG	PSVDWGKIQR	PPEDSIQPYE	KIKARGLPDN
110	120	130	140	150
ISSVLNKLVV	VKLNGGLGTS	MGCKGPKSLI	GVRNENTFLD	LTVQQIEHLN
160	170	180	190	200
KTYNTDVPLV	LMNSFNTDED	TKKILQKYNH	CRVKIYTFNQ	SRYPRINKES
210	220	230	240	250
LLPVAKDVSY	SGENTEAWYP	PGHGDIYASF	YNSGLLDTFI	GEGKEYIFVS
260	270	280	290	300
NIDNLGATVD	LYILNHLMNP	PNGKRCEFVM	EVTNKTRADV	KGGTLTQYEG
310	320	330	340	350
KLRLVEIAQV	PKAHVDEFKS	VSKFKIFNTN	NLWISLAAVK	RLQEQNAIDM
360	370	380	390	400
EIIVNAKTLD	GGLNVIQLET	AVGAAIKSFE	NSLGINVPRS	RFLPVKTTSD
410	420	430	440	450
LLLVMSNLYS	LNAGSLTMSE	KREFPTVPLV	KLGSSFTKVQ	DYLRRFESIP
460	470	480	490	500
DMLELDHLTV	SGDVTFGKNV	SLKGTVIIIA	NHGDRIDIPP	GAVLENKIVS
508
GNLRILDH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-291 Collapse assay details

Data source: Panorama

Official Gene Symbol: UGP2
Peptide Sequence: GLPDNISSVLNK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 96
Peptide End: 107

CPTAC ID: CPTAC-291
Peptide Molecular Mass: 1,255.6772
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	6.4	6.9	5	10.5	7.2	5.3	12.3	10	7.3	15	25	15
y8 (1+)	13.9	10.6	10	13.4	11.7	9.7	19.3	15.8	13.9	15	25	15
y6 (1+)	5.6	8.3	9.8	10.7	9.6	13.4	12.1	12.7	16.6	15	25	15
sum	5.4	7.5	7.3	9.5	8	9	10.9	11	11.6	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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