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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ACSL1 FACL1, FACL2, LACS, LACS1, LACS2
Sequence Length (AA) Molecular Weight (Da)
698 77943
Protein Name
Long-chain-fatty-acid--CoA ligase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQAHELFRYF RMPELVDFRQ YVRTLPTNTL MGFGAFAALT TFWYATRPKP
60 70 80 90 100
LKPPCDLSMQ SVEVAGSGGA RRSALLDSDE PLVYFYDDVT TLYEGFQRGI
110 120 130 140 150
QVSNNGPCLG SRKPDQPYEW LSYKQVAELS ECIGSALIQK GFKTAPDQFI
160 170 180 190 200
GIFAQNRPEW VIIEQGCFAY SMVIVPLYDT LGNEAITYIV NKAELSLVFV
210 220 230 240 250
DKPEKAKLLL EGVENKLIPG LKIIVVMDAY GSELVERGQR CGVEVTSMKA
260 270 280 290 300
MEDLGRANRR KPKPPAPEDL AVICFTSGTT GNPKGAMVTH RNIVSDCSAF
310 320 330 340 350
VKATENTVNP CPDDTLISFL PLAHMFERVV ECVMLCHGAK IGFFQGDIRL
360 370 380 390 400
LMDDLKVLQP TVFPVVPRLL NRMFDRIFGQ ANTTLKRWLL DFASKRKEAE
410 420 430 440 450
LRSGIIRNNS LWDRLIFHKV QSSLGGRVRL MVTGAAPVSA TVLTFLRAAL
460 470 480 490 500
GCQFYEGYGQ TECTAGCCLT MPGDWTAGHV GAPMPCNLIK LVDVEEMNYM
510 520 530 540 550
AAEGEGEVCV KGPNVFQGYL KDPAKTAEAL DKDGWLHTGD IGKWLPNGTL
560 570 580 590 600
KIIDRKKHIF KLAQGEYIAP EKIENIYMRS EPVAQVFVHG ESLQAFLIAI
610 620 630 640 650
VVPDVETLCS WAQKRGFEGS FEELCRNKDV KKAILEDMVR LGKDSGLKPF
660 670 680 690 698
EQVKGITLHP ELFSIDNGLL TPTMKAKRPE LRNYFRSQID DLYSTIKV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-305 Collapse assay details

Data source: Panorama

Official Gene Symbol
ACSL1
Peptide Sequence
IGFFQGDIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
341
Peptide End
349
CPTAC ID
CPTAC-305
Peptide Molecular Mass
1,051.5451
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 18.9 8.2 10.1 24.4 8.5 22.7 30.9 11.8 24.8 15 25 15
y6 (1+) 12.2 7.3 7.6 12 9 12.8 17.1 11.6 14.9 15 25 15
y5 (1+) 13.8 9.2 8.8 13.1 11.3 9.8 19 14.6 13.2 15 25 15
sum 12.5 6.8 4.8 10.7 8.6 6.6 16.5 11 8.2 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-306 Collapse assay details

Data source: Panorama

Official Gene Symbol
ACSL1
Peptide Sequence
VLQPTVFPVVPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
357
Peptide End
368
CPTAC ID
CPTAC-306
Peptide Molecular Mass
1,350.8024
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 8.8 5.9 5.7 11.9 10.9 8 14.8 12.4 9.8 15 25 15
y7 (1+) 19.8 40.3 14.5 69.9 49.8 16.7 72.7 64.1 22.1 15 25 15
y6 (1+) 13.9 5 9.9 14.9 12.6 10.4 20.4 13.6 14.4 15 25 15
sum 10 6.7 6.2 40.2 9.5 6.2 41.4 11.6 8.8 15 25 15

Additional Resources and Comments