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Overview Data source: UniProt

Official Gene Symbol Other Aliases
AIP XAP2
Sequence Length (AA) Molecular Weight (Da)
330 37636
Protein Name
AH receptor-interacting protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MADIIARLRE DGIQKRVIQE GRGELPDFQD GTKATFHYRT LHSDDEGTVL
60 70 80 90 100
DDSRARGKPM ELIIGKKFKL PVWETIVCTM REGEIAQFLC DIKHVVLYPL
110 120 130 140 150
VAKSLRNIAV GKDPLEGQRH CCGVAQMREH SSLGHADLDA LQQNPQPLIF
160 170 180 190 200
HMEMLKVESP GTYQQDPWAM TDEEKAKAVP LIHQEGNRLY REGHVKEAAA
210 220 230 240 250
KYYDAIACLK NLQMKEQPGS PEWIQLDQQI TPLLLNYCQC KLVVEEYYEV
260 270 280 290 300
LDHCSSILNK YDDNVKAYFK RGKAHAAVWN AQEAQADFAK VLELDPALAP
310 320 330
VVSRELQALE ARIRQKDEED KARFRGIFSH

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-308 Collapse assay details

Data source: Panorama

Official Gene Symbol
AIP
Peptide Sequence
VLELDPALAPVVSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
291
Peptide End
304
CPTAC ID
CPTAC-308
Peptide Molecular Mass
1,477.8504
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 31.3 12.4 11.1 33.5 12.9 12.6 45.8 17.9 16.8 15 25 15
y10 (1+) 21.9 21.7 11.7 29 22.6 13.2 36.3 31.3 17.6 15 25 15
y9 (1+) 8.5 7 4.4 11 8.4 6.3 13.9 10.9 7.7 15 25 15
sum 8.8 8.7 5.8 15.7 9.3 5.7 18 12.7 8.1 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-307 Collapse assay details

Data source: Panorama

Official Gene Symbol
AIP
Peptide Modified Sequence
YYDAIAC[+57.0]LK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
208
Peptide - Site of Modification
7
Peptide Start
202
Peptide End
210
CPTAC ID
CPTAC-307
Peptide Molecular Mass
1,115.5321
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6.6 10.5 7.3 12.9 23.8 24.2 14.5 26 25.3 15 25 15
y6 (1+) 16.5 13.7 7.4 16 16.8 21.1 23 21.7 22.4 15 25 15
y5 (1+) 16.3 12.5 16 17.4 11.6 20.6 23.8 17.1 26.1 15 25 15
sum 8.4 9.3 7.4 11 18.1 21.1 13.8 20.3 22.4 15 25 15

Additional Resources and Comments